Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1412958..1413478 Replicon chromosome
Accession NZ_CP080116
Organism Escherichia coli strain Dog168

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KZX73_RS07140 Protein ID WP_001160964.1
Coordinates 1412958..1413242 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KZX73_RS07145 Protein ID WP_000125566.1
Coordinates 1413245..1413478 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX73_RS07115 (1408603) 1408603..1409889 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KZX73_RS07120 (1409889) 1409889..1410878 + 990 WP_094326588.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KZX73_RS07125 (1410875) 1410875..1411696 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KZX73_RS07130 (1411699) 1411699..1412076 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KZX73_RS07135 (1412083) 1412083..1412931 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KZX73_RS07140 (1412958) 1412958..1413242 - 285 WP_001160964.1 CcdB family protein Toxin
KZX73_RS07145 (1413245) 1413245..1413478 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KZX73_RS07150 (1413564) 1413564..1414043 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
KZX73_RS07155 (1414235) 1414235..1416097 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KZX73_RS07160 (1416090) 1416090..1416620 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KZX73_RS07165 (1416728) 1416728..1418059 - 1332 WP_001183209.1 MFS transporter -
KZX73_RS07170 (1418118) 1418118..1418405 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T210797 WP_001160964.1 NZ_CP080116:c1413242-1412958 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T210797 NZ_CP104618:c4167928-4167755 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT210797 WP_000125566.1 NZ_CP080116:c1413478-1413245 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT210797 NZ_CP104618:c4168252-4167956 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References