Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 154646..156269 Replicon plasmid pWW19C-IncHI2
Accession NZ_CP080108
Organism Enterobacter asburiae strain WW-19C

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag KZX48_RS24125 Protein ID WP_000088044.1
Coordinates 154944..156269 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag KZX48_RS24120 Protein ID WP_012477377.1
Coordinates 154646..154939 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX48_RS24100 (KZX48_24090) 150445..150837 + 393 WP_000341066.1 hypothetical protein -
KZX48_RS24105 (KZX48_24095) 151496..152152 + 657 WP_000462754.1 trypsin-like serine protease -
KZX48_RS24110 (KZX48_24100) 152355..152852 + 498 WP_000340139.1 membrane protein -
KZX48_RS24115 (KZX48_24105) 152857..154245 + 1389 WP_000083579.1 hypothetical protein -
KZX48_RS24120 (KZX48_24110) 154646..154939 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
KZX48_RS24125 (KZX48_24115) 154944..156269 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
KZX48_RS24130 (KZX48_24120) 156330..156536 + 207 WP_000134171.1 hypothetical protein -
KZX48_RS24135 (KZX48_24125) 156637..157047 + 411 WP_000985909.1 hypothetical protein -
KZX48_RS24140 (KZX48_24130) 157060..157875 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
KZX48_RS24145 (KZX48_24135) 158128..158553 + 426 WP_000278471.1 hypothetical protein -
KZX48_RS24150 (KZX48_24140) 159102..159410 + 309 WP_001224686.1 hypothetical protein -
KZX48_RS24155 (KZX48_24145) 159426..160283 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / dfrA8 / aac(6')-Ib / ant(3'')-Ia / qacE / sul1 / qnrB10 / ere(A) / blaTEM-1A / blaOXA-9 - 1..332184 332184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T210792 WP_000088044.1 NZ_CP080108:154944-156269 [Enterobacter asburiae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T210792 NZ_CP104618:3388734-3388952 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT210792 WP_012477377.1 NZ_CP080108:154646-154939 [Enterobacter asburiae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT210792 NZ_CP104618:3388334-3388708 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References