Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 701959..702535 Replicon chromosome
Accession NZ_CP080107
Organism Enterobacter asburiae strain WW-19C

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag KZX48_RS03385 Protein ID WP_010427219.1
Coordinates 701959..702246 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag KZX48_RS03390 Protein ID WP_033144548.1
Coordinates 702233..702535 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX48_RS03360 (KZX48_03360) 697059..697754 + 696 WP_094935517.1 winged helix-turn-helix domain-containing protein -
KZX48_RS03365 (KZX48_03365) 697905..698375 + 471 WP_094935516.1 MarR family transcriptional regulator -
KZX48_RS03370 (KZX48_03370) 698372..699439 + 1068 WP_094935515.1 HlyD family secretion protein -
KZX48_RS03375 (KZX48_03375) 699444..700520 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
KZX48_RS03380 (KZX48_03380) 700517..701788 - 1272 WP_094935514.1 DUF445 domain-containing protein -
KZX48_RS03385 (KZX48_03385) 701959..702246 + 288 WP_010427219.1 BrnT family toxin Toxin
KZX48_RS03390 (KZX48_03390) 702233..702535 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
KZX48_RS03395 (KZX48_03395) 702566..703204 - 639 WP_094935513.1 LysE family translocator -
KZX48_RS03400 (KZX48_03400) 703252..703995 - 744 WP_048976422.1 AraC family transcriptional regulator -
KZX48_RS03405 (KZX48_03405) 704147..705517 + 1371 WP_094935512.1 NAD-dependent succinate-semialdehyde dehydrogenase -
KZX48_RS03410 (KZX48_03410) 705564..705884 - 321 WP_039262264.1 hypothetical protein -
KZX48_RS03415 (KZX48_03415) 705884..706429 - 546 WP_023310269.1 YfaZ family outer membrane protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T210777 WP_010427219.1 NZ_CP080107:701959-702246 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T210777 NZ_CP104615:34476-34892 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT210777 WP_033144548.1 NZ_CP080107:702233-702535 [Enterobacter asburiae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT210777 NZ_CP104615:34249-34479 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4R0G892


Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References