Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 199443..200179 Replicon plasmid pWW14A-IncFII/IncR
Accession NZ_CP080100
Organism Klebsiella quasipneumoniae strain WW-14A

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KZX49_RS28060 Protein ID WP_003026803.1
Coordinates 199443..199925 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KZX49_RS28065 Protein ID WP_003026799.1
Coordinates 199913..200179 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX49_RS30035 194601..194783 - 183 Protein_193 ISNCY family transposase -
KZX49_RS30040 194988..195512 - 525 WP_072001637.1 AAA family ATPase -
KZX49_RS28045 (KZX49_28025) 195582..196979 - 1398 WP_032435757.1 ISNCY family transposase -
KZX49_RS28050 (KZX49_28030) 197178..197810 + 633 WP_001567369.1 hypothetical protein -
KZX49_RS28055 (KZX49_28035) 197839..199242 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KZX49_RS28060 (KZX49_28040) 199443..199925 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KZX49_RS28065 (KZX49_28045) 199913..200179 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KZX49_RS28070 (KZX49_28050) 200424..200858 - 435 WP_001567367.1 cell envelope integrity protein TolA -
KZX49_RS28075 (KZX49_28055) 200905..201453 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
KZX49_RS28080 (KZX49_28060) 201905..202255 + 351 WP_004200891.1 hypothetical protein -
KZX49_RS28085 (KZX49_28065) 202685..203665 - 981 WP_000082736.1 IS5-like element ISEc68 family transposase -
KZX49_RS28090 (KZX49_28070) 203724..204179 - 456 Protein_204 DUF932 domain-containing protein -
KZX49_RS28095 (KZX49_28075) 204739..205152 - 414 WP_023280875.1 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..228495 228495
- inside IScluster/Tn - - 183185..203665 20480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T210775 WP_003026803.1 NZ_CP080100:c199925-199443 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T210775 NZ_CP104613:c4448440-4448333 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT210775 WP_003026799.1 NZ_CP080100:c200179-199913 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT210775 NZ_CP104613:4448488-4448554 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References