Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5075..5600 Replicon plasmid pWW14A-IncFII/IncR
Accession NZ_CP080100
Organism Klebsiella quasipneumoniae strain WW-14A

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag KZX49_RS27115 Protein ID WP_013023785.1
Coordinates 5075..5380 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A9E1GDP8
Locus tag KZX49_RS27120 Protein ID WP_004197642.1
Coordinates 5382..5600 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX49_RS27090 (KZX49_27070) 1527..2153 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KZX49_RS30005 2150..2452 + 303 WP_004197636.1 hypothetical protein -
KZX49_RS27100 (KZX49_27080) 2608..2805 + 198 WP_032445779.1 hypothetical protein -
KZX49_RS27105 (KZX49_27085) 2892..3686 - 795 WP_048991183.1 site-specific integrase -
KZX49_RS27110 (KZX49_27090) 3884..4888 - 1005 WP_114266771.1 hypothetical protein -
KZX49_RS27115 (KZX49_27095) 5075..5380 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
KZX49_RS27120 (KZX49_27100) 5382..5600 - 219 WP_004197642.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KZX49_RS27125 (KZX49_27105) 6230..6433 + 204 WP_060579404.1 hypothetical protein -
KZX49_RS27130 (KZX49_27110) 6423..6713 - 291 WP_032433928.1 hypothetical protein -
KZX49_RS27135 (KZX49_27115) 6710..7837 - 1128 WP_060579403.1 DUF4238 domain-containing protein -
KZX49_RS27140 (KZX49_27120) 7871..9394 - 1524 WP_228751153.1 hypothetical protein -
KZX49_RS27145 (KZX49_27125) 9671..10450 - 780 WP_032433925.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..228495 228495


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T210774 WP_013023785.1 NZ_CP080100:c5380-5075 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T210774 NZ_CP104613:c4447957-4447850 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAACGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8376.34 Da        Isoelectric Point: 4.7405

>AT210774 WP_004197642.1 NZ_CP080100:c5600-5382 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT210774 NZ_CP104613:4448005-4448071 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure

References