Detailed information of TA system TA210770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2725383..2726971
• Replicon: chromosome
• Accession: NZ_CP080099
• Organism: Klebsiella quasipneumoniae strain WW-14A

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KZX49_RS13495
• Protein ID: WP_085550860.1
• Coordinates: 2725383..2726705 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: KZX49_RS13500
• Protein ID: WP_023289667.1
• Coordinates: 2726705..2726971 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX49_RS13470 (2720429)	2720429..2722084	-	1656	WP_023289671.1	EAL domain-containing protein	-
KZX49_RS13475 (2722382)	2722382..2722630	+	249	WP_004205912.1	hypothetical protein	-
KZX49_RS13480 (2722922)	2722922..2723140	+	219	WP_002904808.1	ParD-like family protein	-
KZX49_RS13485 (2723121)	2723121..2723903	+	783	WP_023289670.1	type I methionyl aminopeptidase	-
KZX49_RS13490 (2724074)	2724074..2725366	+	1293	WP_139828137.1	glycoside hydrolase family 10 protein	-
KZX49_RS13495 (2725383)	2725383..2726705	-	1323	WP_085550860.1	type II toxin-antitoxin system HipA family toxin	Toxin
KZX49_RS13500 (2726705)	2726705..2726971	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KZX49_RS13505 (2727604)	2727604..2727930	-	327	WP_085550859.1	hydroxyisourate hydrolase	-
KZX49_RS13510 (2727927)	2727927..2728427	-	501	WP_032452825.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KZX49_RS13515 (2728405)	2728405..2729784	-	1380	WP_219804340.1	nucleobase:cation symporter-2 family protein	-
KZX49_RS13520 (2730130)	2730130..2731284	+	1155	WP_085550858.1	FAD-dependent urate hydroxylase HpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2530039..2769118	239079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49253.52 Da
• Isoelectric Point: 8.2665

>T210770 WP_085550860.1 NZ_CP080099:c2726705-2725383 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEETHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRIGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALDLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T210770 NZ_CP104613:4043398-4043862 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT210770 WP_023289667.1 NZ_CP080099:c2726971-2726705 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT210770 NZ_CP104613:4043063-4043398 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3