Detailed information of TA system TA210746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 16863..17455
• Replicon: plasmid pS8245-3
• Accession: NZ_CP080094
• Organism: Salmonella enterica strain SLR1_8245

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: KZY40_RS23470
• Protein ID: WP_000520549.1
• Coordinates: 17207..17455 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: KZY40_RS23465
• Protein ID: WP_000681613.1
• Coordinates: 16863..17210 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY40_RS23435 (KZY40_23435)	12868..13194	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
KZY40_RS23440 (KZY40_23440)	13208..13828	-	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
KZY40_RS23445 (KZY40_23445)	14184..14399	-	216	WP_000760375.1	EexN family lipoprotein	-
KZY40_RS23450 (KZY40_23450)	14396..14689	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
KZY40_RS23455 (KZY40_23455)	14766..15995	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
KZY40_RS23460 (KZY40_23460)	15999..16502	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
KZY40_RS23465 (KZY40_23465)	16863..17210	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KZY40_RS23470 (KZY40_23470)	17207..17455	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
KZY40_RS23475 (KZY40_23475)	17760..18110	-	351	WP_000854259.1	hypothetical protein	-
KZY40_RS23480 (KZY40_23480)	18201..18500	-	300	WP_000650309.1	hypothetical protein	-
KZY40_RS23485 (KZY40_23485)	18954..19181	-	228	WP_000801440.1	hypothetical protein	-
KZY40_RS23490 (KZY40_23490)	19168..19419	-	252	WP_001230707.1	hypothetical protein	-
KZY40_RS23495 (KZY40_23495)	19416..19958	-	543	WP_001083906.1	hypothetical protein	-
KZY40_RS23500 (KZY40_23500)	20063..20578	-	516	Protein_26	DnaJ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33310	33310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T210746 WP_000520549.1 NZ_CP080094:c17455-17207 [Salmonella enterica]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T210746 NZ_CP104605:110684-111109 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT210746 WP_000681613.1 NZ_CP080094:c17210-16863 [Salmonella enterica]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT210746 NZ_CP104605:110457-110687 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1