Detailed information of TA system TA210716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1954127..1954742
• Replicon: chromosome
• Accession: NZ_CP080090
• Organism: Pseudomonas sp. YeP6b

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KZH41_RS08740
• Protein ID: WP_263274687.1
• Coordinates: 1954560..1954742 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZH41_RS08735
• Protein ID: WP_094951024.1
• Coordinates: 1954127..1954537 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZH41_RS08700 (KZH41_08680)	1949193..1950110	+	918	WP_242154873.1	LysR family transcriptional regulator	-
KZH41_RS08705 (KZH41_08685)	1950124..1950534	+	411	WP_242154875.1	fosfomycin resistance glutathione transferase	-
KZH41_RS08710 (KZH41_08690)	1950589..1951230	+	642	WP_094951029.1	LysE family translocator	-
KZH41_RS08715 (KZH41_08695)	1951350..1951877	+	528	WP_094951028.1	DUF4142 domain-containing protein	-
KZH41_RS08720 (KZH41_08700)	1951931..1952356	-	426	WP_094951027.1	low affinity iron permease family protein	-
KZH41_RS08725 (KZH41_08705)	1952534..1953316	+	783	WP_263274685.1	GNAT family N-acetyltransferase	-
KZH41_RS08730 (KZH41_08710)	1953317..1953817	-	501	WP_263274686.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
KZH41_RS08735 (KZH41_08715)	1954127..1954537	-	411	WP_094951024.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KZH41_RS08740 (KZH41_08720)	1954560..1954742	-	183	WP_263274687.1	type II toxin-antitoxin system HicA family toxin	Toxin
KZH41_RS08745 (KZH41_08725)	1954911..1955804	-	894	WP_094951022.1	LysR family transcriptional regulator	-
KZH41_RS08750 (KZH41_08730)	1955900..1957063	+	1164	WP_263274688.1	MFS transporter	-
KZH41_RS08755 (KZH41_08735)	1957060..1958259	+	1200	WP_263274689.1	MFS transporter	-
KZH41_RS08760 (KZH41_08740)	1958244..1958552	-	309	WP_094951019.1	putative addiction module antidote protein	-
KZH41_RS08765 (KZH41_08745)	1958555..1958847	-	293	Protein_1725	type II toxin-antitoxin system RelE/ParE family toxin	-
KZH41_RS08770 (KZH41_08750)	1959163..1959468	-	306	WP_094951018.1	DUF6482 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (7-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6885.15 Da
• Isoelectric Point: 11.4376

>T210716 WP_263274687.1 NZ_CP080090:c1954742-1954560 [Pseudomonas sp. YeP6b]
VDSRYLIGHIVAHGRYLMRTRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKRILKQALLS

Nucleotide

• Length: 183 bp

>T210716 NZ_CP104598:c2472891-2472788 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14775.74 Da
• Isoelectric Point: 4.6121

>AT210716 WP_094951024.1 NZ_CP080090:c1954537-1954127 [Pseudomonas sp. YeP6b]
MLYPIAISTGDENHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPRAQKVTVHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQKEG

Nucleotide

• Length: 411 bp

>AT210716 NZ_CP104598:2472750-2473021 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTATGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT