Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 29360..29882 Replicon plasmid p869Rt_IncX1
Accession NZ_CP080086
Organism Escherichia coli strain 19SZHZ869Rt

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag KZO18_RS23010 Protein ID WP_000220560.1
Coordinates 29360..29641 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KZO18_RS23015 Protein ID WP_000121743.1
Coordinates 29631..29882 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO18_RS22965 (KZO18_22910) 24369..24860 - 492 WP_000872475.1 transcription termination/antitermination NusG family protein -
KZO18_RS22970 (KZO18_22915) 25221..26378 - 1158 WP_000538023.1 DNA distortion polypeptide 2 -
KZO18_RS22975 (KZO18_22920) 26381..26926 - 546 WP_038976855.1 DNA distortion polypeptide 1 -
KZO18_RS22980 (KZO18_22925) 27253..27525 - 273 WP_000160399.1 hypothetical protein -
KZO18_RS22985 (KZO18_22930) 27538..27687 - 150 WP_000003880.1 hypothetical protein -
KZO18_RS22990 (KZO18_22935) 27974..28303 - 330 WP_000866648.1 hypothetical protein -
KZO18_RS22995 (KZO18_22940) 28396..28611 - 216 WP_001180116.1 hypothetical protein -
KZO18_RS23000 (KZO18_22945) 28601..28846 - 246 WP_000356546.1 hypothetical protein -
KZO18_RS23005 (KZO18_22950) 28891..29214 - 324 WP_001181903.1 hypothetical protein -
KZO18_RS23010 (KZO18_22955) 29360..29641 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZO18_RS23015 (KZO18_22960) 29631..29882 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KZO18_RS23020 (KZO18_22965) 31138..31974 + 837 WP_001050931.1 RepB family plasmid replication initiator protein -
KZO18_RS23025 (KZO18_22970) 32014..32460 + 447 WP_001074386.1 hypothetical protein -
KZO18_RS23030 (KZO18_22975) 32597..32950 + 354 WP_000876434.1 DNA distortion polypeptide 3 -
KZO18_RS23035 (KZO18_22980) 33010..33978 - 969 WP_166501310.1 IS5-like element IS903B family transposase -
KZO18_RS23040 (KZO18_22985) 33980..34273 - 294 WP_015060510.1 hypothetical protein -
KZO18_RS23045 (KZO18_22990) 34289..34519 - 231 WP_000051064.1 plasmid partition protein ParG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-12 - 1..36179 36179
- flank IS/Tn - - 33010..33933 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T210715 WP_000220560.1 NZ_CP080086:c29641-29360 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T210715 NZ_CP104598:c2170867-2169545 [Escherichia coli]
ATGCCCAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTACCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACTTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGCTCCAGCGAGGCGCTGAAAGATCGGTATG
ATTTTATGAAATTCCAGGTCTTTCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGGATACACAT
CAGCGATCTCAAACTGGCAATGGGACTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCAGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT210715 WP_000121743.1 NZ_CP080086:c29882-29631 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT210715 NZ_CP104598:c2171133-2170867 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACGGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References