Detailed information of TA system TA210711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4156208..4157045
• Replicon: chromosome
• Accession: NZ_CP080085
• Organism: Escherichia coli strain 19SZHZ869Rt

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KZO18_RS20230
• Protein ID: WP_000227784.1
• Coordinates: 4156208..4156750 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KZO18_RS20235
• Protein ID: WP_001297137.1
• Coordinates: 4156734..4157045 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO18_RS20205 (4151565)	4151565..4152056	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
KZO18_RS20210 (4152184)	4152184..4153548	-	1365	WP_001000978.1	MFS transporter	-
KZO18_RS20215 (4153956)	4153956..4154711	+	756	Protein_3955	tetratricopeptide repeat protein	-
KZO18_RS20220 (4154775)	4154775..4155937	+	1163	WP_085947598.1	IS3-like element IS3 family transposase	-
KZO18_RS20225 (4155961)	4155961..4156152	+	192	Protein_3957	hypothetical protein	-
KZO18_RS20230 (4156208)	4156208..4156750	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KZO18_RS20235 (4156734)	4156734..4157045	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KZO18_RS20240 (4157230)	4157230..4158120	-	891	WP_000971336.1	heme o synthase	-
KZO18_RS20245 (4158132)	4158132..4158461	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KZO18_RS20250 (4158461)	4158461..4159075	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KZO18_RS20255 (4159065)	4159065..4161056	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KZO18_RS20260 (4161078)	4161078..4162025	-	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T210711 WP_000227784.1 NZ_CP080085:c4156750-4156208 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T210711 NZ_CP104598:c1096964-1096746 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT210711 WP_001297137.1 NZ_CP080085:c4157045-4156734 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT210711 NZ_CP104598:c1097364-1096990 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAACTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3