Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2129696..2130350 Replicon chromosome
Accession NZ_CP080085
Organism Escherichia coli strain 19SZHZ869Rt

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZO18_RS10375 Protein ID WP_000244777.1
Coordinates 2129696..2130103 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZO18_RS10380 Protein ID WP_000354046.1
Coordinates 2130084..2130350 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO18_RS10355 (2125653) 2125653..2127386 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
KZO18_RS10360 (2127392) 2127392..2128102 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZO18_RS10365 (2128127) 2128127..2129023 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZO18_RS10370 (2129135) 2129135..2129656 + 522 WP_001055874.1 flavodoxin FldB -
KZO18_RS10375 (2129696) 2129696..2130103 - 408 WP_000244777.1 protein YgfX Toxin
KZO18_RS10380 (2130084) 2130084..2130350 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZO18_RS10385 (2130593) 2130593..2131573 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZO18_RS10390 (2131769) 2131769..2132428 - 660 WP_000250274.1 hemolysin III family protein -
KZO18_RS10395 (2132591) 2132591..2132902 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KZO18_RS10400 (2132947) 2132947..2134380 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
KZO18_RS10405 (2134437) 2134437..2135180 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210704 WP_000244777.1 NZ_CP080085:c2130103-2129696 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210704 NZ_CP104596:29810-30226 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210704 WP_000354046.1 NZ_CP080085:c2130350-2130084 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210704 NZ_CP104596:29583-29813 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References