Detailed information of TA system TA210692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 62271..62872
• Replicon: plasmid p886Rt_IncY
• Accession: NZ_CP080083
• Organism: Escherichia coli strain 19SZHZ886Rt

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: KZO17_RS24415
• Protein ID: WP_001216045.1
• Coordinates: 62271..62651 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KZO17_RS24420
• Protein ID: WP_001190712.1
• Coordinates: 62651..62872 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO17_RS24390 (KZO17_24285)	57711..59153	-	1443	WP_249776110.1	terminase	-
KZO17_RS24395 (KZO17_24290)	59195..60388	-	1194	WP_000219605.1	terminase	-
KZO17_RS24400 (KZO17_24295)	60475..60927	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
KZO17_RS24405 (KZO17_24300)	61016..62059	-	1044	WP_050153511.1	DUF968 domain-containing protein	-
KZO17_RS24410 (KZO17_24305)	62087..62266	-	180	WP_000113019.1	hypothetical protein	-
KZO17_RS24415 (KZO17_24310)	62271..62651	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KZO17_RS24420 (KZO17_24315)	62651..62872	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KZO17_RS24425 (KZO17_24320)	62945..63334	-	390	WP_000506726.1	S24 family peptidase	-
KZO17_RS24430 (KZO17_24325)	63458..63709	-	252	WP_001377386.1	DNA polymerase III subunit theta	-
KZO17_RS24435 (KZO17_24330)	64159..64296	+	138	WP_000123562.1	hypothetical protein	-
KZO17_RS24440 (KZO17_24335)	64371..64733	-	363	WP_001261544.1	hypothetical protein	-
KZO17_RS24445 (KZO17_24340)	64730..65443	-	714	WP_249776116.1	hypothetical protein	-
KZO17_RS24450	65432..65611	-	180	Protein_73	hypothetical protein	-
KZO17_RS24455	65627..65755	-	129	Protein_74	hypothetical protein	-
KZO17_RS24460 (KZO17_24345)	66089..66267	-	179	Protein_75	hypothetical protein	-
KZO17_RS24465 (KZO17_24350)	66269..66457	-	189	WP_000797279.1	hypothetical protein	-
KZO17_RS24470 (KZO17_24355)	66803..67492	-	690	WP_042198318.1	ead/Ea22-like family protein	-
KZO17_RS24475 (KZO17_24360)	67494..67793	-	300	WP_000215167.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93332	93332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T210692 WP_001216045.1 NZ_CP080083:c62651-62271 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T210692 NZ_CP104595:2079510-2079613 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT210692 WP_001190712.1 NZ_CP080083:c62872-62651 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT210692 NZ_CP104595:c2079651-2079380 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6