Detailed information of TA system TA210691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 100409..101052
• Replicon: plasmid p886Rt_hybrid
• Accession: NZ_CP080082
• Organism: Escherichia coli strain 19SZHZ886Rt

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KZO17_RS24040
• Protein ID: WP_063073410.1
• Coordinates: 100636..101052 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3Q0N5I0
• Locus tag: KZO17_RS24035
• Protein ID: WP_063073411.1
• Coordinates: 100409..100639 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO17_RS23995 (95478)	95478..95870	-	393	WP_063073471.1	plasmid partitioning/stability family protein	-
KZO17_RS24000 (95875)	95875..96846	-	972	WP_001103690.1	plasmid segregation protein ParM	-
KZO17_RS24005 (97075)	97075..97719	+	645	WP_000633913.1	division plane positioning ATPase MipZ	-
KZO17_RS24010 (97713)	97713..97988	+	276	WP_050858693.1	hypothetical protein	-
KZO17_RS24015 (98140)	98140..98931	-	792	WP_000016494.1	site-specific integrase	-
KZO17_RS24020 (98928)	98928..99434	-	507	WP_001505085.1	hypothetical protein	-
KZO17_RS24025 (99425)	99425..100129	+	705	WP_001775011.1	IS6-like element IS26 family transposase	-
KZO17_RS24030 (100276)	100276..100452	-	177	Protein_116	hypothetical protein	-
KZO17_RS24035 (100409)	100409..100639	+	231	WP_063073411.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KZO17_RS24040 (100636)	100636..101052	+	417	WP_063073410.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZO17_RS24045 (101126)	101126..102688	+	1563	WP_063073409.1	AAA family ATPase	-
KZO17_RS24050 (102673)	102673..103695	+	1023	WP_063073408.1	hypothetical protein	-
KZO17_RS24055 (103982)	103982..104212	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
KZO17_RS24060 (104209)	104209..104304	+	96	Protein_122	VapC toxin family PIN domain ribonuclease	-
KZO17_RS24065 (104363)	104363..105147	-	785	Protein_123	IS21-like element helper ATPase IstB	-
KZO17_RS24070 (105147)	105147..105800	-	654	Protein_124	IS21-like element IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..110148	110148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15167.53 Da
• Isoelectric Point: 7.8882

>T210691 WP_063073410.1 NZ_CP080082:100636-101052 [Escherichia coli]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCTRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHSIAAGAVLVTNNTREFERVPGLVLEDWIR

Nucleotide

• Length: 417 bp

>T210691 NZ_CP104595:1887646-1887900 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8688.84 Da
• Isoelectric Point: 4.6310

>AT210691 WP_063073411.1 NZ_CP080082:100409-100639 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAKLEKADPDFMAEREDVVTDEGRVNL

Nucleotide

• Length: 231 bp

>AT210691 NZ_CP104595:1887398-1887649 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0N5I0