Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 247185..247827 Replicon chromosome
Accession NC_005957
Organism Bacillus thuringiensis serovar konkukian str. 97-27

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BT9727_RS01390 Protein ID WP_000635963.1
Coordinates 247477..247827 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BT9727_RS01385 Protein ID WP_000004570.1
Coordinates 247185..247472 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BT9727_RS01360 242501..243463 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
BT9727_RS01365 243456..244028 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BT9727_RS01370 244121..244480 + 360 WP_000635040.1 holo-ACP synthase -
BT9727_RS01375 244637..245587 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BT9727_RS01380 245706..246875 + 1170 WP_000390600.1 alanine racemase -
BT9727_RS01385 247185..247472 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BT9727_RS01390 247477..247827 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BT9727_RS01395 247895..250063 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BT9727_RS01400 250121..250237 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BT9727_RS01405 250433..250891 + 459 WP_000344247.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T21069 WP_000635963.1 NC_005957:247477-247827 [[Bacillus thuringiensis] serovar konkukian str. 97-27]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T21069 NC_005957:247477-247827 [[Bacillus thuringiensis] serovar konkukian str. 97-27]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT21069 WP_000004570.1 NC_005957:247185-247472 [[Bacillus thuringiensis] serovar konkukian str. 97-27]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT21069 NC_005957:247185-247472 [[Bacillus thuringiensis] serovar konkukian str. 97-27]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References