Detailed information of TA system TA210687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 35238..35797
• Replicon: plasmid p886Rt_hybrid
• Accession: NZ_CP080082
• Organism: Escherichia coli strain 19SZHZ886Rt

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1V2T4U7
• Locus tag: KZO17_RS23615
• Protein ID: WP_063073486.1
• Coordinates: 35238..35513 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V2T5E1
• Locus tag: KZO17_RS23620
• Protein ID: WP_062894229.1
• Coordinates: 35513..35797 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO17_RS23590 (31036)	31036..32172	-	1137	WP_033804283.1	porin	-
KZO17_RS23595 (32726)	32726..33954	+	1229	WP_167654659.1	IS3 family transposase	-
KZO17_RS23600 (34023)	34023..34212	+	190	Protein_30	IS66 family insertion sequence element accessory protein TnpB	-
KZO17_RS23605 (34215)	34215..34403	-	189	WP_063073487.1	DUF1187 family protein	-
KZO17_RS23610 (34483)	34483..34971	+	489	Protein_32	transposase	-
KZO17_RS23615 (35238)	35238..35513	-	276	WP_063073486.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZO17_RS23620 (35513)	35513..35797	-	285	WP_062894229.1	ribbon-helix-helix domain-containing protein	Antitoxin
KZO17_RS23625 (36708)	36708..37565	-	858	WP_019842754.1	incFII family plasmid replication initiator RepA	-
KZO17_RS23630 (37558)	37558..38040	-	483	WP_074149688.1	hypothetical protein	-
KZO17_RS23635 (38033)	38033..38080	-	48	WP_229471593.1	hypothetical protein	-
KZO17_RS23640 (38071)	38071..38322	+	252	WP_223195197.1	replication protein RepA	-
KZO17_RS23645 (38339)	38339..38596	-	258	WP_000083845.1	replication regulatory protein RepA	-
KZO17_RS23650 (38862)	38862..39722	-	861	WP_000027057.1	broad-spectrum class A beta-lactamase TEM-1	-
KZO17_RS23655 (39905)	39905..40462	-	558	WP_001235713.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..110148	110148
	-	inside	IScluster/Tn	blaTEM-1B	-	25134..43631	18497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10724.40 Da
• Isoelectric Point: 9.8136

>T210687 WP_063073486.1 NZ_CP080082:c35513-35238 [Escherichia coli]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIYAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T210687 NZ_CP104595:929517-929924 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10486.95 Da
• Isoelectric Point: 5.6558

>AT210687 WP_062894229.1 NZ_CP080082:c35797-35513 [Escherichia coli]
MQMKNNTAQVTKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT210687 NZ_CP104595:929270-929536 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1V2T4U7

Antitoxin

• AlphaFold DB: A0A1V2T5E1