Detailed information of TA system TA210685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 100021..100543
• Replicon: plasmid p886Rt_247k_tetX
• Accession: NZ_CP080081
• Organism: Escherichia coli strain 19SZHZ886Rt

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: KZO17_RS22650
• Protein ID: WP_000220560.1
• Coordinates: 100262..100543 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: KZO17_RS22645
• Protein ID: WP_000121743.1
• Coordinates: 100021..100272 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO17_RS22620 (KZO17_22555)	95670..95951	+	282	WP_001185482.1	hypothetical protein	-
KZO17_RS22625 (KZO17_22560)	95976..96953	+	978	WP_024245156.1	hypothetical protein	-
KZO17_RS22630 (KZO17_22565)	96950..97306	-	357	WP_000456533.1	DNA distortion polypeptide 3	-
KZO17_RS22635 (KZO17_22570)	97443..97889	-	447	Protein_106	hypothetical protein	-
KZO17_RS22640 (KZO17_22575)	97929..98765	-	837	WP_001050931.1	RepB family plasmid replication initiator protein	-
KZO17_RS22645 (KZO17_22580)	100021..100272	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
KZO17_RS22650 (KZO17_22585)	100262..100543	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZO17_RS22655 (KZO17_22590)	100618..101315	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
KZO17_RS22660 (KZO17_22595)	101647..102033	-	387	WP_063102497.1	bleomycin binding protein	-
KZO17_RS22665 (KZO17_22600)	102227..102931	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(X4) / floR / tet(A) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / qnrS1 / blaTEM-1B / lnu(G)	-	1..246552	246552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T210685 WP_000220560.1 NZ_CP080081:100262-100543 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T210685 NZ_CP104595:c603421-602957 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT210685 WP_000121743.1 NZ_CP080081:100021-100272 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT210685 NZ_CP104595:c603756-603421 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301