Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 12247..12769 | Replicon | plasmid p663Rt_52k_tetX |
| Accession | NZ_CP080077 | ||
| Organism | Escherichia coli strain 19SZHZ663Rt | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | KZO16_RS23550 | Protein ID | WP_000220560.1 |
| Coordinates | 12488..12769 (+) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | KZO16_RS23545 | Protein ID | WP_000121743.1 |
| Coordinates | 12247..12498 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZO16_RS23515 (KZO16_23445) | 7610..7840 | + | 231 | WP_000051064.1 | plasmid partition protein ParG | - |
| KZO16_RS23520 (KZO16_23450) | 7856..8149 | + | 294 | WP_015060510.1 | hypothetical protein | - |
| KZO16_RS23525 (KZO16_23455) | 8151..9119 | + | 969 | WP_166501310.1 | IS5-like element IS903B family transposase | - |
| KZO16_RS23530 (KZO16_23460) | 9179..9532 | - | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
| KZO16_RS23535 (KZO16_23465) | 9669..10115 | - | 447 | WP_001074386.1 | hypothetical protein | - |
| KZO16_RS23540 (KZO16_23470) | 10155..10991 | - | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
| KZO16_RS23545 (KZO16_23475) | 12247..12498 | + | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| KZO16_RS23550 (KZO16_23480) | 12488..12769 | + | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KZO16_RS23555 (KZO16_23485) | 12915..13238 | + | 324 | WP_001181903.1 | hypothetical protein | - |
| KZO16_RS23560 (KZO16_23490) | 13283..13528 | + | 246 | WP_000356546.1 | hypothetical protein | - |
| KZO16_RS23565 (KZO16_23495) | 13518..13733 | + | 216 | WP_001180116.1 | hypothetical protein | - |
| KZO16_RS23570 (KZO16_23500) | 13826..14155 | + | 330 | WP_000866648.1 | hypothetical protein | - |
| KZO16_RS23575 (KZO16_23505) | 14442..14591 | + | 150 | WP_000003880.1 | hypothetical protein | - |
| KZO16_RS23580 (KZO16_23510) | 14604..14876 | + | 273 | WP_000160399.1 | hypothetical protein | - |
| KZO16_RS23585 (KZO16_23515) | 15203..15748 | + | 546 | WP_038976855.1 | DNA distortion polypeptide 1 | - |
| KZO16_RS23590 (KZO16_23520) | 15751..16908 | + | 1158 | WP_000538023.1 | DNA distortion polypeptide 2 | - |
| KZO16_RS23595 (KZO16_23525) | 17269..17760 | + | 492 | WP_000872475.1 | transcription termination/antitermination NusG family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | lnu(F) / aadA2 / blaSHV-12 / tet(A) / floR / tet(X4) | - | 1..51975 | 51975 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T210665 WP_000220560.1 NZ_CP080077:12488-12769 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T210665 NZ_CP104587:c387136-386759 [Pseudomonas aeruginosa]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT210665 WP_000121743.1 NZ_CP080077:12247-12498 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT210665 NZ_CP104587:c386778-386443 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |