Detailed information of TA system TA021066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-pasA/PRK09812-HTH
• Location: 818049..818716
• Replicon: chromosome
• Accession: NC_005956
• Organism: Bartonella henselae str. Houston-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: X5M710
• Locus tag: AYT27_RS03650
• Protein ID: WP_011180620.1
• Coordinates: 818049..818381 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: N6VJG4
• Locus tag: AYT27_RS03655
• Protein ID: WP_007346764.1
• Coordinates: 818489..818716 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYT27_RS03605	813167..813646	+	480	WP_011180611.1	hypothetical protein	-
AYT27_RS03610	813636..814886	+	1251	WP_011180612.1	DUF1376 domain-containing protein	-
AYT27_RS03615	814870..815256	+	387	WP_082246570.1	hypothetical protein	-
AYT27_RS03620	815437..815757	+	321	Protein_674	hypothetical protein	-
AYT27_RS03625	815945..816217	-	273	WP_011180615.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
AYT27_RS03630	816214..816504	-	291	WP_011180616.1	hypothetical protein	-
AYT27_RS03635	816716..817162	+	447	WP_011180617.1	single-stranded DNA-binding protein	-
AYT27_RS03640	817216..817749	+	534	WP_011180618.1	hypothetical protein	-
AYT27_RS03645	817791..818049	+	259	Protein_679	antitoxin	-
AYT27_RS03650	818049..818381	+	333	WP_011180620.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYT27_RS03655	818489..818716	+	228	WP_007346764.1	ribbon-helix-helix protein, CopG family	Antitoxin
AYT27_RS03660	818700..818969	+	270	WP_011180622.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AYT27_RS03665	818999..819388	-	390	WP_011180623.1	hypothetical protein	-
AYT27_RS03670	819487..819945	-	459	WP_011180624.1	hypothetical protein	-
AYT27_RS03680	820244..820570	+	327	WP_011180626.1	hypothetical protein	-
AYT27_RS03685	820551..821690	+	1140	WP_049784545.1	phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	806463..821690	15227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Protein of unknown function DUF6290 (2-71)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11865.88 Da
• Isoelectric Point: 10.5590

>T21066 WP_011180620.1 NC_005956:818049-818381 [Bartonella henselae str. Houston-1]
MKRGEIWLVSLDPTSGYEQKGTRPVLIVSPEAFNRVTKTPIVLPITSGGNFSRTAGFAVSLVGTGLHTTGVIRCDQPRAL
DIAARQGKKMETVPPIIMNEVLAKLSTFLT

Nucleotide

• Length: 333 bp

>T21066 NC_005956:818049-818381 [Bartonella henselae str. Houston-1]
ATGAAGCGGGGCGAAATTTGGCTTGTATCTCTTGACCCAACTTCAGGCTATGAACAAAAAGGAACGCGTCCTGTATTGAT
TGTATCACCAGAGGCGTTTAATCGTGTCACGAAAACACCCATTGTCTTACCCATTACAAGTGGAGGAAATTTTTCTCGAA
CCGCAGGTTTCGCTGTCTCATTGGTAGGAACAGGATTGCATACAACAGGTGTGATACGTTGTGACCAGCCACGTGCTCTG
GATATAGCAGCGCGTCAGGGTAAGAAAATGGAAACAGTTCCCCCCATAATTATGAATGAAGTTCTTGCTAAGCTATCAAC
ATTTCTCACATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8742.78 Da
• Isoelectric Point: 4.8780

>AT21066 WP_007346764.1 NC_005956:818489-818716 [Bartonella henselae str. Houston-1]
MTISIRLPSDLETRLNNLAAKTGRTKSFYLREIIERGIEEAEDYYLASQVRERVRRGEGTFYSSEEVRKELGLDD

Nucleotide

• Length: 228 bp

>AT21066 NC_005956:818489-818716 [Bartonella henselae str. Houston-1]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Structures

Toxin

• AlphaFold DB: A0A067W4K9

Antitoxin

• AlphaFold DB: N6VJG4