Detailed information of TA system TA210655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3458856..3459693
• Replicon: chromosome
• Accession: NZ_CP080075
• Organism: Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KZO16_RS16565
• Protein ID: WP_000227784.1
• Coordinates: 3459151..3459693 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KZO16_RS16560
• Protein ID: WP_001297137.1
• Coordinates: 3458856..3459167 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO16_RS16535 (3453876)	3453876..3454823	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KZO16_RS16540 (3454845)	3454845..3456836	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KZO16_RS16545 (3456826)	3456826..3457440	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KZO16_RS16550 (3457440)	3457440..3457769	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KZO16_RS16555 (3457781)	3457781..3458671	+	891	WP_000971336.1	heme o synthase	-
KZO16_RS16560 (3458856)	3458856..3459167	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KZO16_RS16565 (3459151)	3459151..3459693	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KZO16_RS16570 (3459749)	3459749..3460684	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
KZO16_RS16575 (3461092)	3461092..3462456	+	1365	WP_001000974.1	MFS transporter	-
KZO16_RS16580 (3462584)	3462584..3463075	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KZO16_RS16585 (3463243)	3463243..3464154	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T210655 WP_000227784.1 NZ_CP080075:3459151-3459693 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T210655 NZ_CP104584:c5870184-5869906 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT210655 WP_001297137.1 NZ_CP080075:3458856-3459167 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT210655 NZ_CP104584:c5869894-5869589 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3