Detailed information of TA system TA210641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1298138..1298763
• Replicon: chromosome
• Accession: NZ_CP080075
• Organism: Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZO16_RS06250
• Protein ID: WP_000911330.1
• Coordinates: 1298365..1298763 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KZO16_RS06245
• Protein ID: WP_000450524.1
• Coordinates: 1298138..1298365 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO16_RS06220 (1293941)	1293941..1294411	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KZO16_RS06225 (1294411)	1294411..1294983	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KZO16_RS06230 (1295129)	1295129..1296007	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KZO16_RS06235 (1296024)	1296024..1297058	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
KZO16_RS06240 (1297271)	1297271..1297984	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KZO16_RS06245 (1298138)	1298138..1298365	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZO16_RS06250 (1298365)	1298365..1298763	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZO16_RS06255 (1298910)	1298910..1299773	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
KZO16_RS06260 (1299788)	1299788..1301803	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
KZO16_RS06265 (1301877)	1301877..1302575	+	699	WP_000679812.1	esterase	-
KZO16_RS06270 (1302656)	1302656..1302856	-	201	WP_124053586.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T210641 WP_000911330.1 NZ_CP080075:1298365-1298763 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T210641 NZ_CP104583:19237-19533 [Pseudomonas sp. GD03721]
GTGCCTAGCTTCAAGCTTGAGTTTCACGTCAAAGCCCGCAAGGAATGGGACAAGCTCGACGCGACCATTCGCCTCCAGTT
TGCCAAAAAGCTTAAAGAGCGCCTGAGCTCGCCTCGCATTGAGGCCGACAAGCTCAGTGGCATGGTTGACTGCTACAAGA
TCAAGCTTCGCTCTGCTGGCTACCGGCTTGTTTATCAGGTTGTCGATGACAAACTGGTTATAACGGTTGTGGCCGTTGGT
AAGCGTGAACGCTTTGAGGCCTACATTGCCGCTCAAAAACGAGTACAGCAGCAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT210641 WP_000450524.1 NZ_CP080075:1298138-1298365 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT210641 NZ_CP104583:18966-19244 [Pseudomonas sp. GD03721]
ATGACCTTTCCAGTGCTGGCTGACGTCGCCGCTTCCGTTACTGACTTCAAGCGTGATCCGATGGGCACCATTCGCGAAGG
GCACGGTGAAACCGTCGTCATCCTCAACCGTAACGAACCGGCCTTTTACGCCGTGCCTCCAGCCCGCTATCAAGCCATGC
TCGAAATGCTCGATGACTTACGGCTGGCCGAAATTGTGCGTGCTCGCCAGGGGGGCGAAACAGTCTCGGTAGATATTGAC
GATCTGATCGCAGAAGCCGAGGCTGGCATCCGTGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33