Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1003168..1003751 Replicon chromosome
Accession NZ_CP080075
Organism Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KZO16_RS04825 Protein ID WP_000254745.1
Coordinates 1003416..1003751 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KZO16_RS04820 Protein ID WP_000581937.1
Coordinates 1003168..1003416 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO16_RS04810 (999507) 999507..1000808 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KZO16_RS04815 (1000856) 1000856..1003090 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KZO16_RS04820 (1003168) 1003168..1003416 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KZO16_RS04825 (1003416) 1003416..1003751 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KZO16_RS04830 (1003822) 1003822..1004613 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KZO16_RS04835 (1004841) 1004841..1006478 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KZO16_RS04840 (1006566) 1006566..1007864 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T210640 WP_000254745.1 NZ_CP080075:1003416-1003751 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T210640 NZ_CP104583:9618-9905 [Pseudomonas sp. GD03721]
ATGACCTATAGCCTCGAATTCGATGCGCGGGCACTGAAGGAGTGGCACAAGCTGGGGGATACCGTCCGCGAGCAGTTCAA
AAAGAAGCTCGCGGAGGTGTTGGTCAATCCGCGCGTTGAGGCAAATCGCCTTCACTCACTTCCCGACTGCTACAAGATCA
AACTGCGCAGCAGCGGTTATCGCCTGGTGTACCAGGTGATCGACCATGAGGTGGTGGTATTTGTCGTGGCCGTGGATCGG
CGTGATCGCGAGCAGGCTTACCGCAAGGCCGCTGAGCAGCTCAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT210640 WP_000581937.1 NZ_CP080075:1003168-1003416 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT210640 NZ_CP104583:9389-9628 [Pseudomonas sp. GD03721]
ATGGCTTCGATCAATATCCGCATCGACGACGAGCTGAAAGTCCGCGCATACCGGGAACTGGAGAAGCTTGGCGTCACCCC
TTCTGAGCTGATGCGCCAAACCCTGCAATACGTGGCCGAGCGGGGTCAGCTGCCTTTCCGCCCGGTACTGATGACCGAGG
AAGATGAGGCCCTGATTGCCACTGTGTGTGAGCGCCTTGCTGCTCCCCAGCGCGTGAAGGTCTCGCTGGATGACCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References