Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 866833..867487 Replicon chromosome
Accession NZ_CP080075
Organism Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KZO16_RS04210 Protein ID WP_000244772.1
Coordinates 867080..867487 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZO16_RS04205 Protein ID WP_000354046.1
Coordinates 866833..867099 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO16_RS04180 (862121) 862121..862864 + 744 WP_000951964.1 SDR family oxidoreductase -
KZO16_RS04185 (862921) 862921..864354 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
KZO16_RS04190 (864399) 864399..864710 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KZO16_RS04195 (864874) 864874..865533 + 660 WP_000250274.1 hemolysin III family protein -
KZO16_RS04200 (865610) 865610..866590 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZO16_RS04205 (866833) 866833..867099 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZO16_RS04210 (867080) 867080..867487 + 408 WP_000244772.1 protein YgfX Toxin
KZO16_RS04215 (867527) 867527..868048 - 522 WP_001055874.1 flavodoxin FldB -
KZO16_RS04220 (868160) 868160..869056 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZO16_RS04225 (869081) 869081..869791 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZO16_RS04230 (869797) 869797..871530 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T210639 WP_000244772.1 NZ_CP080075:867080-867487 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210639 NZ_CP104582:c2497388-2497137 [Pseudomonas sp. GD03919]
ATGCGCACTATCAGCTATACCGAAGCCCGGCAGAACCTGTCGGCCACCATGGTGCAGACCGTCGAGGATCGCGTGCCTAC
CCTCATTACCCGCCAGAACGGCGAGGCGTGCGTGCTGATGTCGCTGGAGGAGTACAACGCGCTGGAGGAAACGGCTTACC
TGCTGCGCTCGCCGGCCAACGCGCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCGTGGCCAGGGTGTCGAGCGGGAT
ATCGCCGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210639 WP_000354046.1 NZ_CP080075:866833-867099 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210639 NZ_CP104582:c2497140-2496886 [Pseudomonas sp. GD03919]
GTGAAGCTGATCTGGTCTGAGCAAGCCTGGGACGATTATCTGTACTGGCAAGAGGCGGATAAGCGTATGGTCAAGAAGCT
CAACGAGCTGATCAAGGAGACCCGCCGCACCCCTTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGGCGGATCACCGCCGAGCACCGGTTTGTGTATTCGGTGACGGACGAGGCGCTGCTGATTGCCGCCTGC
CGTTATCACTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References