Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 296262..296893 Replicon chromosome
Accession NZ_CP080075
Organism Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag KZO16_RS01330 Protein ID WP_001259388.1
Coordinates 296262..296537 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KZO16_RS01335 Protein ID WP_000593555.1
Coordinates 296534..296893 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO16_RS01315 (291266) 291266..292333 + 1068 WP_000361485.1 HlyD family secretion protein -
KZO16_RS01320 (292330) 292330..295065 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
KZO16_RS01325 (295065) 295065..296189 + 1125 WP_001314210.1 ABC transporter permease -
KZO16_RS01330 (296262) 296262..296537 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
KZO16_RS01335 (296534) 296534..296893 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZO16_RS01340 (297013) 297013..297414 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KZO16_RS01345 (297420) 297420..298226 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KZO16_RS01350 (298223) 298223..298987 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KZO16_RS01355 (298987) 298987..299820 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KZO16_RS01360 (299817) 299817..300761 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 279066..296893 17827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T210637 WP_001259388.1 NZ_CP080075:296262-296537 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T210637 NZ_CP104582:2388024-2388320 [Pseudomonas sp. GD03919]
ATGGAAAAGCGCAAACCCCATTTCAAGCTTGAGCTGGTGAAGCAGGCCATAACCGAGCAGCGTTATCGCTTCACCCGCGT
AGCTTTGGAAGGGGGCGCCGAGCTGGGTATGGAGATGGCTGACATGCTGGCCGTTATCAGTGCCCTGAGCAGCCGCGACT
TTTTCAAGAGCATTACAACCTATGCGGATCATACTACCTGGCAGGACGTTTACCGACCCGATAACGAGTTCGGGCAGGTC
TACTTGAAGTTCACGCTGGTGGCTGATCTGCTGATCGTTTCCTTCAAGGAGAAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT210637 WP_000593555.1 NZ_CP080075:296534-296893 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT210637 NZ_CP104582:2388323-2388724 [Pseudomonas sp. GD03919]
ATGAAATGTCCAGTATGCGGCGGGGCGGAACTGGTTCACGACACCCGTGATATGCCCTTCACCTACAAGGGCCAGACCAC
CCAAATTACTGCAGTAACGGCTGACTGGTGCGATGCCTGTGGCGAGTCCCTGACTGGGCCGGCTGAGAGTGAGCACGTTA
TGCGTGCCATGAACGAGTTTCGCCAGCAGGTGAACGCGCAGGACGGTAATCAGGAGCTGATTCGCAGTGTGCGTAAGCAG
TTGCGGTTGAGCCAGCGCGAGGCCGCCGAGCTATTCGGTGGTGGCCCGAATGCGTTTTCTCGCTACGAGCGAGGCAGCAC
AGAAGCACCGCAACCGCTGGTGCAGTTGTTCAAGATCCTGGGCCGACACCCGGAGTTGATTAACGAGCTGCGTGCAGGTT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References