Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 921413..922067 Replicon chromosome
Accession NZ_CP080070
Organism Escherichia coli strain 19SZHZ713Rt

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZO15_RS04465 Protein ID WP_000244777.1
Coordinates 921660..922067 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZO15_RS04460 Protein ID WP_000354046.1
Coordinates 921413..921679 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO15_RS04435 (916582) 916582..917325 + 744 WP_000951962.1 SDR family oxidoreductase -
KZO15_RS04440 (917382) 917382..918815 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
KZO15_RS04445 (918860) 918860..919171 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KZO15_RS04450 (919335) 919335..919994 + 660 WP_000250274.1 hemolysin III family protein -
KZO15_RS04455 (920190) 920190..921170 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZO15_RS04460 (921413) 921413..921679 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZO15_RS04465 (921660) 921660..922067 + 408 WP_000244777.1 protein YgfX Toxin
KZO15_RS04470 (922107) 922107..922628 - 522 WP_001055874.1 flavodoxin FldB -
KZO15_RS04475 (922740) 922740..923636 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZO15_RS04480 (923661) 923661..924371 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZO15_RS04485 (924377) 924377..926110 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210620 WP_000244777.1 NZ_CP080070:921660-922067 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210620 NZ_CP104579:c4513376-4513122 [Pseudomonas oleovorans]
GTGAATCTGGTGTTCTCCGAGCAAGCCTGGGCGGATTACCTCTACTGGCAAGGGCAGGACAAGAAGACCCTGAAACGGAT
CAACGAACTGCTCAAGGAAATCAAAAGAACGCCATTCGAGGGCATGGGCAAACCCGAACCGCTCAAGCACGCCCTGGCGG
GCTACTGGTCGCGGCGGATCAACGAAGAGCATCGTCTGGTGTACAGGCTCCGAGAGCATGAAATTCTGATCGCCCAGGTG
CGTTATCACTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210620 WP_000354046.1 NZ_CP080070:921413-921679 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210620 NZ_CP104579:c4513624-4513373 [Pseudomonas oleovorans]
ATGGACGCCATCACCTACAGCACCGCACGCGCCAACCTGGCCAATACCATGGATCGCGTATGCGAGGATCACGAACCCAT
CATCATCACCCGCAATGGTCAGCAGGCTGTGGTGATGATGTCGCTCGACGACTACAAGGCACTGGAAGAAACCGCCTACC
TGCTGCGTAGCCCTAAGAACGCACAGCGTCTGCTCGAAAGTATCGCCGCGCTTGAGACGGGCAAGGGAGTCACGCGAGAG
CTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References