Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4257171..4257668 Replicon chromosome
Accession NZ_CP080066
Organism Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KZO14_RS20370 Protein ID WP_001173343.1
Coordinates 4257171..4257344 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KZO14_RS20375 Protein ID WP_000398619.1
Coordinates 4257372..4257668 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO14_RS20360 (4253841) 4253841..4255298 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
KZO14_RS20365 (4255535) 4255535..4257052 + 1518 WP_001295074.1 lysine--tRNA ligase -
KZO14_RS20370 (4257171) 4257171..4257344 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KZO14_RS20375 (4257372) 4257372..4257668 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KZO14_RS20380 (4257895) 4257895..4258167 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KZO14_RS20385 (4258179) 4258179..4258409 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KZO14_RS20390 (4258590) 4258590..4260221 + 1632 WP_001216486.1 sensor histidine kinase -
KZO14_RS20395 (4260218) 4260218..4260937 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T210614 WP_001173343.1 NZ_CP080066:c4257344-4257171 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T210614 NZ_CP104579:c2089730-2089449 [Pseudomonas oleovorans]
ATGACCTATAAGCTCGAATTCCTGCCCTCAGCGCTGAAGGAATGGGAGAAGCTGGGTCATACGGTGCGTGAGCAGGCAAA
GAAAAAACTGGGTGAGCGGCTGGAAGCACCAAAGGTACAGGCTGATGCCCTCAGGGATCTGCCTGGGCACTACAAGATCA
AGCTCCGAACAGCAAGCTATCGATTGGTATATCGGGTCGAAGATGATCGGGTGGTTGTGATGGTGGTCGCAGTGGGCAAG
CGTGAACGAAGTACAGCTTACAAGGCAGCAGGTAAGCGTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT210614 WP_000398619.1 NZ_CP080066:c4257668-4257372 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT210614 NZ_CP104579:c2089965-2089720 [Pseudomonas oleovorans]
ATGCAGAGTGTTTTGGCTGACGTTGCCGTGAGCGTTTCTGAGCTGAAGAAAAACCCCACTGGCGTCATGGCTGGTGCTGG
GGGCATGCCGGTCGCCGTTCTCAATCATAACCGCGTGATGGGGTACATGGTGCCCGCCGAGCTGTACGAGCAGATGCTGG
AACGTCTGGACGATCTGGATCTGATCGATGTCGTTAACGCACGCTCCGGTGAGAAGGGTGTGCCGGTAAACCTGGATGAC
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References