Detailed information of TA system TA210611

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3673882..3674585
• Replicon: chromosome
• Accession: NZ_CP080066
• Organism: Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8H9SVB9
• Locus tag: KZO14_RS17600
• Protein ID: WP_072003801.1
• Coordinates: 3673882..3674247 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8H9SW74
• Locus tag: KZO14_RS17605
• Protein ID: WP_032250315.1
• Coordinates: 3674268..3674585 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO14_RS17580 (3669803)	3669803..3670027	-	225	Protein_3450	DUF3987 domain-containing protein	-
KZO14_RS17585 (3670133)	3670133..3670360	-	228	WP_039022145.1	AlpA family phage regulatory protein	-
KZO14_RS17590 (3671052)	3671052..3672689	+	1638	WP_039025855.1	hypothetical protein	-
KZO14_RS17595 (3672776)	3672776..3673372	-	597	WP_047643993.1	hypothetical protein	-
KZO14_RS17600 (3673882)	3673882..3674247	-	366	WP_072003801.1	TA system toxin CbtA family protein	Toxin
KZO14_RS17605 (3674268)	3674268..3674585	-	318	WP_032250315.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZO14_RS17610 (3674604)	3674604..3674825	-	222	Protein_3456	DUF987 domain-containing protein	-
KZO14_RS17615 (3674834)	3674834..3675316	-	483	WP_032250314.1	DNA repair protein RadC	-
KZO14_RS17620 (3675325)	3675325..3675783	-	459	WP_032250313.1	antirestriction protein	-
KZO14_RS17625 (3675886)	3675886..3676050	-	165	Protein_3459	DUF905 family protein	-
KZO14_RS17630 (3676384)	3676384..3678048	-	1665	WP_032250311.1	ATP-dependent helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3652286..3698145	45859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13583.80 Da
• Isoelectric Point: 8.8806

>T210611 WP_072003801.1 NZ_CP080066:c3674247-3673882 [Escherichia coli]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLKKHYGLTLNDTPFCDQTVIQEHIDAGITLANAVNFLVEKHELVRIDRTGF
TSQEQAPYLTVTDILHARRACGLMNSCSYREVSNIVLSRSR

Nucleotide

• Length: 366 bp

>T210611 NZ_CP104579:c748293-747997 [Pseudomonas oleovorans]
ATGGCTGAAATAGTCTGGACGAACACCGCCCTCGAGCAGCTCGACGATCTCGCGCACTACATCGCCCTCGACAAACCGGA
CGCCGCGCGAGCGCTGGTCAGGAGGGCCGTTGAAACGGTTTCACGGTTAGCAGACTTTCCCCTGTCGGGCCGCGTTCCGG
ATGAGCTTCCACACTCGGTGTACCGCGAAATCGTCATCCCACCCTGCCGAATTTTCTACCGCTATACAGACACAACGGTT
TTCATCATCCACATCATGCGAGAGGAGCGCGTGCTGCGCGCCCACATGCTCGAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11873.59 Da
• Isoelectric Point: 6.8407

>AT210611 WP_032250315.1 NZ_CP080066:c3674585-3674268 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPQFISQMESMLTTGEMNPRHAHCVILYH
NGFTCEADTFGSCGYVYIAIYPIQR

Nucleotide

• Length: 318 bp

>AT210611 NZ_CP104579:c748538-748281 [Pseudomonas oleovorans]
ATGAAAGTCGAGTTGGTCACCAACCTGAAGCGACAGGCCACCAAGATTCTGGCCGACCTTCACGAGTCCAAAGAACCTGT
ACTGATCACCGAGCATGGAATGCCTTCGGCTTATCTGGTGGATGTCGATGACTACCAGTTCATGCAGAGGCGTCTTGCCC
TTCTGGATGCCCTGGCTCGCGGAGAACGTGCCGTAATCGAGGGCAACACCCAGAACCATGAGCAGGCCAAGGAACGGCTG
AGCAAATGGCTGAAATAG