Detailed information of TA system TA210609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3457232..3458069
• Replicon: chromosome
• Accession: NZ_CP080066
• Organism: Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KZO14_RS16630
• Protein ID: WP_000227784.1
• Coordinates: 3457527..3458069 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KZO14_RS16625
• Protein ID: WP_001297137.1
• Coordinates: 3457232..3457543 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO14_RS16600 (3452252)	3452252..3453199	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KZO14_RS16605 (3453221)	3453221..3455212	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KZO14_RS16610 (3455202)	3455202..3455816	+	615	WP_000179810.1	cytochrome o ubiquinol oxidase subunit III	-
KZO14_RS16615 (3455816)	3455816..3456145	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KZO14_RS16620 (3456157)	3456157..3457047	+	891	WP_000971336.1	heme o synthase	-
KZO14_RS16625 (3457232)	3457232..3457543	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KZO14_RS16630 (3457527)	3457527..3458069	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KZO14_RS16635 (3458125)	3458125..3459060	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
KZO14_RS16640 (3459468)	3459468..3460832	+	1365	WP_001000975.1	MFS transporter	-
KZO14_RS16645 (3460960)	3460960..3461451	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KZO14_RS16650 (3461619)	3461619..3462530	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T210609 WP_000227784.1 NZ_CP080066:3457527-3458069 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T210609 NZ_CP104562:c2882506-2882024 [Roseateles sp. BIM B-1768]
TTGATCACCGCCGCGCATGATTGCTCGGCCTTCACCTGCACCCATACCCCGCTGACTGATTGGCTGCAGCGCCGCGCACT
GCCCAATCAGGCGGCCGGCGCATCGCGGACCTATGTGGTCAGCACCAGCGATCAGCAGGTTGTCGGCTACTACGCCCTGG
CGCCGGGTGCGGTGGCTGCGGACGCCACACCCGGTGCCCTGCGCCGCAACATGCCGCAACCCATTCCGGTGTTCGTGCTG
GGTCGGCTGGCGGTGCATTCAGCCTGGCAAGGCCTGGGCATCGGCAGCGGACTGCTGCGGGACGCGGTGCTGCGCAGCGC
CGAGGCCGCTCAGATCGTCGGGGGCCGCGCCCTGCTGTGCCATTCCATCGATGAGCAGGCCAAGGGGTTCTACTGCAAGC
ACGGCTTTGTCCAGTCCCCGATGGAGCCGCTGACCGTCATGCTGGGCCTCAAAGCATCATCGACCGCACCGCCCAACGCC
TGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT210609 WP_001297137.1 NZ_CP080066:3457232-3457543 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT210609 NZ_CP104562:c2882802-2882533 [Roseateles sp. BIM B-1768]
ATGACGGCATCTACCACCACCATCAACTTCCGAGCCACCACGGACTTCCGCGATCTGATCGATCGCGCTGCCACCGCCCA
AGGCAAGACCCGCACGGACTTCATCATGGCCGCGTCGGAGGCGGAGGCACAGCGCGTGCTGCTGGACCGCGCCTTCTTCC
AACTGGACGAAGCGCAGATGCAGGCCTTCCAGGCCGTGCTGGACCAGCCGATCGACCAGAACGCGGCCGTCAATCGGTTG
CTCGCGAAAAAAGCGCCATGGGATCAATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3