Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3423155..3423773 Replicon chromosome
Accession NZ_CP080066
Organism Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZO14_RS16460 Protein ID WP_001291435.1
Coordinates 3423555..3423773 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZO14_RS16455 Protein ID WP_000344800.1
Coordinates 3423155..3423529 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO14_RS16445 (3418244) 3418244..3419437 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZO14_RS16450 (3419460) 3419460..3422609 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KZO14_RS16455 (3423155) 3423155..3423529 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZO14_RS16460 (3423555) 3423555..3423773 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZO14_RS16465 (3423944) 3423944..3424495 + 552 WP_000102578.1 maltose O-acetyltransferase -
KZO14_RS16470 (3424611) 3424611..3425081 + 471 WP_000136192.1 YlaC family protein -
KZO14_RS16475 (3425245) 3425245..3426795 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZO14_RS16480 (3426837) 3426837..3427190 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KZO14_RS16490 (3427569) 3427569..3427880 + 312 WP_000409911.1 MGMT family protein -
KZO14_RS16495 (3427911) 3427911..3428483 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210608 WP_001291435.1 NZ_CP080066:3423555-3423773 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210608 NZ_CP104562:75480-75857 [Roseateles sp. BIM B-1768]
ATGAACATCGTGGTGGACACCTCGGTATGGATCGACTTTTTCCGCGCCGGCAATGACCGCTTGCTGCATCTGCTGGACGC
CGACGTCGTGCTTGCGCACCCGATGATTATTGGGGAGCTGGCCTGCGGCACGCCACGGTCCCCCAGGACGGCGTCGCTGA
GTATGCTCCGTCGTCTCGGGCTCAGCCGTGCGGTGGCGATGGAGGAGGTCCTCAGACTGGTCGAGCAGGAGAAGCTCTAC
GGCAAGGGCTGCGGACTGGTGGACATGGTGGTCCTGGCCTCCACCCTCAAGACGCCAGGCGCGGTGCTGTGGTCGCTGGA
CAAGCGGTTGACCCTGCTTGCAGAGCAGTTCCAGATCGCCATGCCAAGCGTGCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210608 WP_000344800.1 NZ_CP080066:3423155-3423529 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210608 NZ_CP104562:75277-75483 [Roseateles sp. BIM B-1768]
ATGGAGATGACCATCATGATTGACGAAGCGCTGTACCGGGAGGCCCTCGAAGTGGTCGGCCGGCCCATGCCGGTCGACGA
ACTCGTAACCACGGCACTGAGAACCTTCATCCGGGTCGAGGCCGGCCGGCGTCTGGCCGCACTGGGCGGCACCGCGCCGG
ACATGCCAGACATTCCTCGCCGAAGAGAGGCGCCAGACGAATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References