Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1039244..1039827 Replicon chromosome
Accession NZ_CP080066
Organism Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KZO14_RS05070 Protein ID WP_000254745.1
Coordinates 1039492..1039827 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KZO14_RS05065 Protein ID WP_000581937.1
Coordinates 1039244..1039492 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO14_RS05055 (1035583) 1035583..1036884 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KZO14_RS05060 (1036932) 1036932..1039166 + 2235 WP_000226828.1 GTP diphosphokinase -
KZO14_RS05065 (1039244) 1039244..1039492 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KZO14_RS05070 (1039492) 1039492..1039827 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KZO14_RS05075 (1039898) 1039898..1040689 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KZO14_RS05080 (1040917) 1040917..1042554 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KZO14_RS05085 (1042642) 1042642..1043940 + 1299 WP_000036723.1 phosphopyruvate hydratase -
KZO14_RS05090 (1043996) 1043996..1044358 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T210596 WP_000254745.1 NZ_CP080066:1039492-1039827 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T210596 NZ_CP104557:336351-336575 [Pseudomonas promysalinigenes]
CTGGGGGATACGGTTCGGCAGCAACTCAAAAGAAAACTCGCCTCGGTTTTGCTTAATCCGAGGGTTGAGGCAAACCGGCT
GGATAGCTTGCCGAACGGCTACAAGATAAAGCTGCGCAGTAGCGGCTACCGATTGATTTACCAGGTGGTCGATCAGGAGG
AGACATCAGAAGTCCTCCGCGTTGCTGCGCGGCAGTTTTACGCGCTTGCTGGTATTGGCTTCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT210596 WP_000581937.1 NZ_CP080066:1039244-1039492 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT210596 NZ_CP104557:336071-336310 [Pseudomonas promysalinigenes]
ATGGCATCCATCAATGTTCGTATCGACGACGATCTCAAAATCCGAGCCTACCGTGAGCTCGAGCGGCTGGGTGTTACGCC
CTCCGAACTCATGCGCCAAGCCTTGCAGTACGTCGCTGAGCGCGGGCAATTGCCTTTCAAGCCTGTGCTCATGACGGATG
AAGATGAGGCGCTGCTGGCGACCGTTCGCGAGCGCCTTGCCGACCCTCAGCGAGTGAAGGTGTCGCTGGATGACCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References