Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 902738..903392 Replicon chromosome
Accession NZ_CP080066
Organism Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZO14_RS04460 Protein ID WP_000244777.1
Coordinates 902985..903392 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZO14_RS04455 Protein ID WP_000354046.1
Coordinates 902738..903004 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO14_RS04430 (897907) 897907..898650 + 744 WP_000951948.1 SDR family oxidoreductase -
KZO14_RS04435 (898707) 898707..900140 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KZO14_RS04440 (900185) 900185..900496 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KZO14_RS04445 (900660) 900660..901319 + 660 WP_000250274.1 hemolysin III family protein -
KZO14_RS04450 (901515) 901515..902495 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZO14_RS04455 (902738) 902738..903004 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZO14_RS04460 (902985) 902985..903392 + 408 WP_000244777.1 protein YgfX Toxin
KZO14_RS04465 (903432) 903432..903953 - 522 WP_001055870.1 flavodoxin FldB -
KZO14_RS04470 (904065) 904065..904961 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZO14_RS04475 (904986) 904986..905696 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZO14_RS04480 (905702) 905702..907435 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210595 WP_000244777.1 NZ_CP080066:902985-903392 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210595 NZ_CP104555:871387-871686 [Vibrio sp. J502]
ATGAAACCATTCCAACTAACCAACAAGGCAAAATCCGATTTAAAAGATATTGCCTTATTTACCTCTCGCAGGTGGGGCCG
CGAGCAACGAAATATTTATCTCAAACAATTCGATGAATCATTTTGGCTACTCGCTGAAAATCCTGATATCGGTAAAACTT
GTGATGAAATCAGAGATGGCTACCGAAAATTTCCACAAGGAAGCCATGTTATTTTTTATCAACAAATTGGTAGCCAAAAC
ATCCAGATCATAAGAATCTTGCATAAGAGTATGGATGTAAATCCAATCTTTGGCGCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210595 WP_000354046.1 NZ_CP080066:902738-903004 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210595 NZ_CP104555:871148-871390 [Vibrio sp. J502]
ATGGCTAAAAATACTAGCATCACTCTCGGCGACCACTTTGATGGTTTCATTAGTAGCCAAATCCAAAGTGGACGCTATGG
CTCAGCAAGTGAAGTAATTCGTTCCGCTCTACGTTTACTCGAAACACAAGAAACGAAAATGAACACTTTACGACAACTGC
TCGTTGAAGGTGAAGAAAGTGGTATGGCTGATTATGACCTCAATTCGTTTATCAGTGAACTAGATAGTGAAGAAAGAAAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References