Detailed information of TA system TA210593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 337288..338088
• Replicon: chromosome
• Accession: NZ_CP080066
• Organism: Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: KZO14_RS01615
• Protein ID: WP_000342449.1
• Coordinates: 337561..338088 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: KZO14_RS01610
• Protein ID: WP_001277108.1
• Coordinates: 337288..337554 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO14_RS01590 (332946)	332946..333614	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
KZO14_RS01595 (333607)	333607..334665	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
KZO14_RS01600 (334910)	334910..335764	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
KZO14_RS01605 (336035)	336035..337138	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
KZO14_RS01610 (337288)	337288..337554	+	267	WP_001277108.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
KZO14_RS01615 (337561)	337561..338088	+	528	WP_000342449.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
KZO14_RS01620 (338085)	338085..338468	-	384	WP_000778785.1	aspartate 1-decarboxylase autocleavage activator PanM	-
KZO14_RS01625 (338892)	338892..340001	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
KZO14_RS01630 (340049)	340049..340975	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KZO14_RS01635 (340972)	340972..342249	+	1278	WP_063122063.1	branched chain amino acid ABC transporter permease LivM	-
KZO14_RS01640 (342246)	342246..343013	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T210593 WP_000342449.1 NZ_CP080066:337561-338088 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T210593 NZ_CP104555:853757-854035 [Vibrio sp. J502]
ATGATTTTATGGGAAGAAGAATCGCTAAATGATCGTGAGAAGATCTTTGAGTTTCTTTATGACTTTAACCCTGATGCGGC
TGAAAAAACTGATGACATTATTGAGGCTAAAGTAGAAAACTTACTCGAACAACCATTGATGGGTGTTCAACGGGATGGAA
TTCGAGGACGATTGCTCATTATTCCCGAAATCTCAATGATCGTTTCGTACTGGGTAGAAGGTTCAATTATTCGCATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT210593 WP_001277108.1 NZ_CP080066:337288-337554 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT210593 NZ_CP104555:853476-853760 [Vibrio sp. J502]
ATGGACACTAGAATTCAATTTCGTGTTGATGACGAAATTAAACGTTTAGCCCAACAAATGGCTGAGAGCCAAGGCCGCAC
ACTCAGCGATGCTTGCCGTGAACTTACTGAACAAATGGCTGAGCAACAAAGAAAAACATTATCTCATGACGCTTGGTTAA
CTGAACAAATAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTTCATTTGTTGAACATAATTCAGCTAAAGCACGA
ATGGCTGAACGAAAGGCCAAAATTCGTAATCGAGGCCAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24