Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 54674..55275 Replicon plasmid p655Rt_p0111
Accession NZ_CP080059
Organism Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KZO13_RS24470 Protein ID WP_001216045.1
Coordinates 54674..55054 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KZO13_RS24475 Protein ID WP_001190712.1
Coordinates 55054..55275 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO13_RS24440 49774..49893 - 120 WP_071594056.1 ash family protein -
KZO13_RS24445 (KZO13_24420) 50115..51599 - 1485 WP_096321054.1 terminase -
KZO13_RS24450 (KZO13_24425) 51599..52792 - 1194 WP_096321055.1 terminase -
KZO13_RS24455 (KZO13_24430) 52878..53330 - 453 WP_001326849.1 late promoter-activating protein -
KZO13_RS24460 (KZO13_24435) 53419..54462 - 1044 WP_069187067.1 DUF968 domain-containing protein -
KZO13_RS24465 (KZO13_24440) 54490..54669 - 180 WP_001339207.1 hypothetical protein -
KZO13_RS24470 (KZO13_24445) 54674..55054 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZO13_RS24475 (KZO13_24450) 55054..55275 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZO13_RS24480 (KZO13_24455) 55458..57014 + 1557 WP_249768529.1 type I restriction-modification system subunit M -
KZO13_RS24485 (KZO13_24460) 57011..58231 + 1221 WP_144339994.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94084 94084


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T210591 WP_001216045.1 NZ_CP080059:c55054-54674 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T210591 NZ_CP104555:c844683-844429 [Vibrio sp. J502]
ATGAGAATTGTATCTTTTACAGAAGCTAGAAATAGCTTGAAGAGCGTTTTGGATGCTGTTGTTAATGACGCAGATACAAC
CGTGATCACACGTCGTGATTCTGAAGATGCCGTTGTTATGTCACTGGATTATTACAATAGCTTAATGGAAACGGTTCATT
TACTTCGTTCTCCGGCAAATGCTGAGCACTTACATCGTTCAATTGCTCAATTTAAAGCGGGTAAGGCGAGCCAAAGAGAT
TTAATCGATGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT210591 WP_001190712.1 NZ_CP080059:c55275-55054 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT210591 NZ_CP104555:c844436-844173 [Vibrio sp. J502]
ATGAGTAGTCGTTTATTATCTTGGACTGATGAATCTTGGAATGATTATGTTTATTGGCAGACGCAAGACAAGAAAACACT
TAAACGTATAAATAAACTCATCACGGATGCTAAGCGTTCTCCTTTCGAGGGCATAGGTAAACCTGAGCCACTAAAAGAAA
ACTTATCTGGTTTTTGGTCGCGACGAATAGACGATGCGAATAGGTTAGTTTATGCGGTTGATGATAGTGCTATCACGATT
ATTTCTTGCCGTTATCACTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References