Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 60019..60541 Replicon plasmid p655Rt_hybrid
Accession NZ_CP080058
Organism Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag KZO13_RS24005 Protein ID WP_000220560.1
Coordinates 60260..60541 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KZO13_RS24000 Protein ID WP_000121743.1
Coordinates 60019..60270 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO13_RS23975 (KZO13_23960) 55690..55971 + 282 WP_001185482.1 hypothetical protein -
KZO13_RS23980 (KZO13_23965) 55996..56973 + 978 WP_024245156.1 hypothetical protein -
KZO13_RS23985 (KZO13_23970) 56970..57326 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
KZO13_RS23990 (KZO13_23975) 57463..57909 - 447 Protein_72 hypothetical protein -
KZO13_RS23995 (KZO13_23980) 57949..58785 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
KZO13_RS24000 (KZO13_23985) 60019..60270 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KZO13_RS24005 (KZO13_23990) 60260..60541 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZO13_RS24010 (KZO13_23995) 60616..61313 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
KZO13_RS24015 (KZO13_24000) 61323..61631 + 309 Protein_77 replication initiation protein -
KZO13_RS24020 (KZO13_24005) 62923..63891 + 969 WP_030005799.1 IS5 family transposase -
KZO13_RS24025 (KZO13_24010) 64031..64417 - 387 WP_001039464.1 plasmid stabilization protein StbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid erm(42) / floR / tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(6)-Id / aph(3'')-Ib - 1..104551 104551


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T210590 WP_000220560.1 NZ_CP080058:60260-60541 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T210590 NZ_CP104555:c844436-844173 [Vibrio sp. J502]
ATGAGTAGTCGTTTATTATCTTGGACTGATGAATCTTGGAATGATTATGTTTATTGGCAGACGCAAGACAAGAAAACACT
TAAACGTATAAATAAACTCATCACGGATGCTAAGCGTTCTCCTTTCGAGGGCATAGGTAAACCTGAGCCACTAAAAGAAA
ACTTATCTGGTTTTTGGTCGCGACGAATAGACGATGCGAATAGGTTAGTTTATGCGGTTGATGATAGTGCTATCACGATT
ATTTCTTGCCGTTATCACTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT210590 WP_000121743.1 NZ_CP080058:60019-60270 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT210590 NZ_CP104555:c844683-844429 [Vibrio sp. J502]
ATGAGAATTGTATCTTTTACAGAAGCTAGAAATAGCTTGAAGAGCGTTTTGGATGCTGTTGTTAATGACGCAGATACAAC
CGTGATCACACGTCGTGATTCTGAAGATGCCGTTGTTATGTCACTGGATTATTACAATAGCTTAATGGAAACGGTTCATT
TACTTCGTTCTCCGGCAAATGCTGAGCACTTACATCGTTCAATTGCTCAATTTAAAGCGGGTAAGGCGAGCCAAAGAGAT
TTAATCGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References