Detailed information of TA system TA210589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 9480..10027
• Replicon: plasmid p655Rt_hybrid
• Accession: NZ_CP080058
• Organism: Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KZO13_RS23700
• Protein ID: WP_244578848.1
• Coordinates: 9480..9758 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A829FT18
• Locus tag: KZO13_RS23705
• Protein ID: WP_000079938.1
• Coordinates: 9758..10027 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO13_RS23665 (KZO13_23660)	5146..7054	+	1909	Protein_7	Tn3-like element Tn3 family transposase	-
KZO13_RS23670 (KZO13_23665)	7089..7622	+	534	Protein_8	helix-turn-helix domain-containing protein	-
KZO13_RS23675 (KZO13_23670)	7631..8056	+	426	WP_063072980.1	ester cyclase	-
KZO13_RS23680 (KZO13_23675)	8251..8355	+	105	Protein_10	IS3 family transposase	-
KZO13_RS23685 (KZO13_23680)	8659..8952	+	294	WP_223429961.1	biofilm development regulator YmgB/AriR family protein	-
KZO13_RS23690	9077..9213	-	137	Protein_12	IS5/IS1182 family transposase	-
KZO13_RS23695 (KZO13_23685)	9290..9427	-	138	WP_152931508.1	hypothetical protein	-
KZO13_RS23700 (KZO13_23690)	9480..9758	-	279	WP_244578848.1	type II toxin-antitoxin system toxin YacB	Toxin
KZO13_RS23705 (KZO13_23695)	9758..10027	-	270	WP_000079938.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
KZO13_RS23710 (KZO13_23700)	10100..10240	-	141	WP_160784086.1	hypothetical protein	-
KZO13_RS23715 (KZO13_23705)	10751..11096	-	346	Protein_17	IS1 family transposase	-
KZO13_RS23720 (KZO13_23710)	11365..12120	-	756	WP_000807690.1	replication initiation protein RepE	-
KZO13_RS23725 (KZO13_23715)	12654..12818	+	165	Protein_19	IS1 family transposase	-
KZO13_RS23730 (KZO13_23720)	12883..13587	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KZO13_RS23735 (KZO13_23725)	13593..13928	+	336	WP_223413452.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	erm(42) / floR / tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(6)-Id / aph(3'')-Ib	-	1..104551	104551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.39 Da
• Isoelectric Point: 8.4615

>T210589 WP_244578848.1 NZ_CP080058:c9758-9480 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMVAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSPWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T210589 NZ_CP104555:786395-786694 [Vibrio sp. J502]
ATGCACAAGAGTAAATATAAACTAAGTAAATTAGCACAGGCTCATTTACATAAAATCAAAAATTATACCGTTACAAACTT
TTCCGAGATGCAGTGGAACGCATACAAAGACACTCTTCTTACTGGGTTTCAGATGCTCGCTGACAACCCCGCTGTAGGCA
GAAGCTGCAACGATTTATATCAAAATGGTTTTTACTTCCCTATAGGCAAGCATACGGCTTACTTTACCAAGGAAGACAGT
TTTATTTTGGTCGTTGCAGTCCTTGGGCAATCGCAATTGCCACAAAACCATCTGCGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 10.1734

>AT210589 WP_000079938.1 NZ_CP080058:c10027-9758 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT210589 NZ_CP104555:786145-786387 [Vibrio sp. J502]
ATGTACACATTAACAGCAAATGATGCCAAACGTAATTTCGGTGAACTGCTTCTAAACGCTCAACGTGAGCCAGTTAAAAT
TAGTAAAAACAATAAAGATGCCGTTGTTGTGATGTCGATTAGAGACTATGAAGAACTTGAAGTCATGAAAGCCGATTACA
TAAAACATTGTTTTGAGTCCGCAAAAGCAGACTTAGCCCAAGGCAATATAGTTGACGGTGAAAACTTCCTAAACGCGTTG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A829FT18