Detailed information of TA system TA210583

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3625520..3626354
• Replicon: chromosome
• Accession: NZ_CP080056
• Organism: Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X7CAN1
• Locus tag: KZO13_RS17305
• Protein ID: WP_039025883.1
• Coordinates: 3625520..3625897 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2X5LBZ2
• Locus tag: KZO13_RS17310
• Protein ID: WP_039025882.1
• Coordinates: 3625986..3626354 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO13_RS17275 (3620778)	3620778..3621734	+	957	WP_000121354.1	molybdenum cofactor insertion chaperone PaoD	-
KZO13_RS17280 (3621785)	3621785..3622123	+	339	Protein_3387	LysR substrate-binding domain-containing protein	-
KZO13_RS17285 (3622461)	3622461..3623780	+	1320	WP_000144688.1	site-specific integrase	-
KZO13_RS17290 (3623873)	3623873..3624721	-	849	WP_039025884.1	DUF4942 domain-containing protein	-
KZO13_RS17295 (3624818)	3624818..3625015	-	198	WP_000772024.1	DUF957 domain-containing protein	-
KZO13_RS17300 (3625035)	3625035..3625523	-	489	Protein_3391	DUF5983 family protein	-
KZO13_RS17305 (3625520)	3625520..3625897	-	378	WP_039025883.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KZO13_RS17310 (3625986)	3625986..3626354	-	369	WP_039025882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZO13_RS17315 (3626404)	3626404..3627048	-	645	WP_039025881.1	hypothetical protein	-
KZO13_RS17320 (3627063)	3627063..3627284	-	222	WP_000692312.1	DUF987 domain-containing protein	-
KZO13_RS17325 (3627353)	3627353..3627829	-	477	WP_001186742.1	RadC family protein	-
KZO13_RS17330 (3627844)	3627844..3628329	-	486	WP_063073032.1	antirestriction protein	-
KZO13_RS17335 (3628420)	3628420..3629238	-	819	WP_001234702.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3595955..3636681	40726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14176.22 Da
• Isoelectric Point: 7.8523

>T210583 WP_039025883.1 NZ_CP080056:c3625897-3625520 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T210583 NZ_CP104551:c4834378-4834094 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13792.52 Da
• Isoelectric Point: 5.9582

>AT210583 WP_039025882.1 NZ_CP080056:c3626354-3625986 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT210583 NZ_CP104551:c4834610-4834368 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2X7CAN1

Antitoxin

• AlphaFold DB: A0A2X5LBZ2