Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3435206..3435824 Replicon chromosome
Accession NZ_CP080056
Organism Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZO13_RS16400 Protein ID WP_001291435.1
Coordinates 3435606..3435824 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZO13_RS16395 Protein ID WP_000344800.1
Coordinates 3435206..3435580 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO13_RS16385 (3430295) 3430295..3431488 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZO13_RS16390 (3431511) 3431511..3434660 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KZO13_RS16395 (3435206) 3435206..3435580 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZO13_RS16400 (3435606) 3435606..3435824 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KZO13_RS16405 (3435996) 3435996..3436547 + 552 WP_000102564.1 maltose O-acetyltransferase -
KZO13_RS16410 (3436663) 3436663..3437133 + 471 WP_001402072.1 YlaC family protein -
KZO13_RS16415 (3437297) 3437297..3438847 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZO13_RS16420 (3438889) 3438889..3439242 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KZO13_RS16430 (3439621) 3439621..3439932 + 312 WP_000409911.1 MGMT family protein -
KZO13_RS16435 (3439963) 3439963..3440535 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-69)

Antitoxin

(1-117)


Sequences


Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210582 WP_001291435.1 NZ_CP080056:3435606-3435824 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210582 NZ_CP104551:c4731334-4730801 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210582 WP_000344800.1 NZ_CP080056:3435206-3435580 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210582 NZ_CP104551:c4731611-4731345 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References