Detailed information of TA system TA210570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1382484..1383109
• Replicon: chromosome
• Accession: NZ_CP080056
• Organism: Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZO13_RS06645
• Protein ID: WP_000911330.1
• Coordinates: 1382711..1383109 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KZO13_RS06640
• Protein ID: WP_000450524.1
• Coordinates: 1382484..1382711 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO13_RS06615 (1378287)	1378287..1378757	-	471	WP_001402398.1	thioredoxin-dependent thiol peroxidase	-
KZO13_RS06620 (1378757)	1378757..1379329	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KZO13_RS06625 (1379475)	1379475..1380353	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KZO13_RS06630 (1380370)	1380370..1381404	+	1035	WP_001491244.1	outer membrane protein assembly factor BamC	-
KZO13_RS06635 (1381617)	1381617..1382330	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KZO13_RS06640 (1382484)	1382484..1382711	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZO13_RS06645 (1382711)	1382711..1383109	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZO13_RS06650 (1383256)	1383256..1384119	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
KZO13_RS06655 (1384134)	1384134..1386149	+	2016	WP_136655682.1	tRNA cytosine(34) acetyltransferase TmcA	-
KZO13_RS06660 (1386223)	1386223..1386921	+	699	WP_000679812.1	esterase	-
KZO13_RS06665 (1387031)	1387031..1387231	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T210570 WP_000911330.1 NZ_CP080056:1382711-1383109 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T210570 NZ_CP104546:1862587-1862769 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT210570 WP_000450524.1 NZ_CP080056:1382484-1382711 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT210570 NZ_CP104546:1862871-1863272 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33