Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 897589..898243 Replicon chromosome
Accession NZ_CP080056
Organism Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZO13_RS04375 Protein ID WP_000244777.1
Coordinates 897836..898243 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZO13_RS04370 Protein ID WP_000354046.1
Coordinates 897589..897855 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO13_RS04345 (892758) 892758..893501 + 744 WP_000951964.1 SDR family oxidoreductase -
KZO13_RS04350 (893558) 893558..894991 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KZO13_RS04355 (895036) 895036..895347 + 312 WP_001402516.1 N(4)-acetylcytidine aminohydrolase -
KZO13_RS04360 (895511) 895511..896170 + 660 WP_000250274.1 hemolysin III family protein -
KZO13_RS04365 (896366) 896366..897346 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZO13_RS04370 (897589) 897589..897855 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZO13_RS04375 (897836) 897836..898243 + 408 WP_000244777.1 protein YgfX Toxin
KZO13_RS04380 (898283) 898283..898804 - 522 WP_001402515.1 flavodoxin FldB -
KZO13_RS04385 (898916) 898916..899812 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZO13_RS04390 (899837) 899837..900547 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZO13_RS04395 (900553) 900553..902286 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210568 WP_000244777.1 NZ_CP080056:897836-898243 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210568 NZ_CP104542:c40163-39882 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210568 WP_000354046.1 NZ_CP080056:897589-897855 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210568 NZ_CP104542:c40404-40153 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References