Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 295786..296372 Replicon chromosome
Accession NZ_CP080056
Organism Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KZO13_RS01330 Protein ID WP_042014162.1
Coordinates 295786..296016 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KZO13_RS01335 Protein ID WP_000593555.1
Coordinates 296013..296372 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO13_RS01320 (291823) 291823..294558 + 2736 WP_001402601.1 ribosome-associated ATPase/putative transporter RbbA -
KZO13_RS01325 (294558) 294558..295682 + 1125 WP_001314210.1 ABC transporter permease -
KZO13_RS01330 (295786) 295786..296016 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KZO13_RS01335 (296013) 296013..296372 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZO13_RS01340 (296492) 296492..296893 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KZO13_RS01345 (296899) 296899..297705 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KZO13_RS01350 (297702) 297702..298466 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KZO13_RS01355 (298466) 298466..299299 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KZO13_RS01360 (299296) 299296..300240 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T210566 WP_042014162.1 NZ_CP080056:295786-296016 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T210566 NZ_CP104541:c35759-35454 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATTCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT210566 WP_000593555.1 NZ_CP080056:296013-296372 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT210566 NZ_CP104541:c35979-35761 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAATGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References