Detailed information of TA system TA210564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4832815..4833488
• Replicon: chromosome
• Accession: NZ_CP080029
• Organism: Streptomyces anulatus strain VUW1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZO11_RS21270
• Protein ID: WP_219611549.1
• Coordinates: 4832815..4833249 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7H0NJE1
• Locus tag: KZO11_RS21275
• Protein ID: WP_073800431.1
• Coordinates: 4833246..4833488 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO11_RS21255 (KZO11_21245)	4827868..4830729	+	2862	WP_219611547.1	FAD-binding and (Fe-S)-binding domain-containing protein	-
KZO11_RS21260 (KZO11_21250)	4830739..4831629	-	891	WP_219611548.1	LysR family transcriptional regulator	-
KZO11_RS21265 (KZO11_21255)	4831809..4832780	+	972	WP_050361183.1	EamA family transporter	-
KZO11_RS21270 (KZO11_21260)	4832815..4833249	-	435	WP_219611549.1	PIN domain-containing protein	Toxin
KZO11_RS21275 (KZO11_21265)	4833246..4833488	-	243	WP_073800431.1	CopG family transcriptional regulator	Antitoxin
KZO11_RS21280 (KZO11_21270)	4833571..4834011	-	441	Protein_4209	short-chain dehydrogenase	-
KZO11_RS21285 (KZO11_21275)	4834259..4834750	+	492	WP_257895204.1	DinB family protein	-
KZO11_RS21290 (KZO11_21280)	4834881..4835687	+	807	WP_219611551.1	TIGR03084 family metal-binding protein	-
KZO11_RS21295 (KZO11_21285)	4835684..4837426	+	1743	WP_219611552.1	acyclic terpene utilization AtuA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-40)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 16042.30 Da
• Isoelectric Point: 4.6667

>T210564 WP_219611549.1 NZ_CP080029:c4833249-4832815 [Streptomyces anulatus]
MIIVADTSALYAAFDAAQTEHSEAARILENERLAISPLVVTELDHLVHRDLGFPAAMQVMEALNSRMGDGQYRLAELKLA
DLRTAQEVRQKYEGLRLDLADAVGVVLADRYRTDRIFTLDQRDFRAMSPLTPGLDAFRILPADG

Nucleotide

• Length: 435 bp

>T210564 NZ_CP104540:106419-106817 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9158.38 Da
• Isoelectric Point: 5.8769

>AT210564 WP_073800431.1 NZ_CP080029:c4833488-4833246 [Streptomyces anulatus]
MIKTTVYLPEELEVRLDAESSATGVSKAELIRRSIALLLDSAERPKRTRQLPVFDSGRSLTPDEMDDSVYEHIKERNARR

Nucleotide

• Length: 243 bp

>AT210564 NZ_CP104540:106192-106419 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H0NJE1