Detailed information of TA system TA210562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 2595464..2596100
• Replicon: chromosome
• Accession: NZ_CP080028
• Organism: Heyndrickxia oleronia strain DSM 9356

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0Q3WTD1
• Locus tag: KZP24_RS13185
• Protein ID: WP_055741345.1
• Coordinates: 2595750..2596100 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8E2I5A7
• Locus tag: KZP24_RS13180
• Protein ID: WP_058005316.1
• Coordinates: 2595464..2595745 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZP24_RS13160	2591517..2592107	-	591	WP_078111169.1	rhomboid family intramembrane serine protease	-
KZP24_RS13165	2592185..2592538	+	354	WP_058005314.1	holo-ACP synthase	-
KZP24_RS13170	2592743..2593759	+	1017	WP_058005315.1	outer membrane lipoprotein carrier protein LolA	-
KZP24_RS13175	2594119..2595294	+	1176	WP_078111168.1	alanine racemase	-
KZP24_RS13180	2595464..2595745	+	282	WP_058005316.1	hypothetical protein	Antitoxin
KZP24_RS13185	2595750..2596100	+	351	WP_055741345.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KZP24_RS13190	2596631..2597461	+	831	WP_058005317.1	RsbT co-antagonist protein RsbRA	-
KZP24_RS13195	2597452..2597814	+	363	WP_139254832.1	STAS domain-containing protein	-
KZP24_RS13200	2597819..2598220	+	402	WP_058005319.1	anti-sigma regulatory factor	-
KZP24_RS13205	2598232..2599239	+	1008	WP_078109472.1	PP2C family protein-serine/threonine phosphatase	-
KZP24_RS13210	2599315..2599644	+	330	WP_058005321.1	anti-sigma factor antagonist	-
KZP24_RS13215	2599644..2600117	+	474	WP_058005322.1	anti-sigma B factor RsbW	-
KZP24_RS13220	2600095..2600892	+	798	WP_078109473.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8998

>T210562 WP_055741345.1 NZ_CP080028:2595750-2596100 [Heyndrickxia oleronia]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMEKVDDALQISLGLIEF

Nucleotide

• Length: 351 bp

>T210562 NZ_CP104540:c93931-93644 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10774.26 Da
• Isoelectric Point: 6.4777

>AT210562 WP_058005316.1 NZ_CP080028:2595464-2595745 [Heyndrickxia oleronia]
VSESSATTEILIRLPVHLLSELDGFAEQENVNRNEFIYRATKMYLRERKKRQIRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT210562 NZ_CP104540:c94179-93928 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A0Q3WTD1