Detailed information of TA system TA210556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5618658..5619310
• Replicon: chromosome
• Accession: NZ_CP080011
• Organism: Pseudomonas aeruginosa strain TL3773

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZH79_RS25875
• Protein ID: WP_003134109.1
• Coordinates: 5618900..5619310 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KZH79_RS25870
• Protein ID: WP_170869361.1
• Coordinates: 5618658..5618900 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZH79_RS25855 (KZH79_25855)	5613666..5615555	-	1890	WP_191519263.1	hypothetical protein	-
KZH79_RS25860 (KZH79_25860)	5615667..5616419	-	753	WP_191519262.1	hypothetical protein	-
KZH79_RS25865 (KZH79_25865)	5616427..5618352	-	1926	WP_191519261.1	type I DNA topoisomerase	-
KZH79_RS25870 (KZH79_25870)	5618658..5618900	+	243	WP_170869361.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KZH79_RS25875 (KZH79_25875)	5618900..5619310	+	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KZH79_RS25880 (KZH79_25880)	5619326..5619487	+	162	WP_191519260.1	hypothetical protein	-
KZH79_RS25885 (KZH79_25885)	5619537..5619755	+	219	WP_023083218.1	hypothetical protein	-
KZH79_RS25890 (KZH79_25890)	5619821..5620018	-	198	WP_191519259.1	CrpP family ICE-associated protein	-
KZH79_RS25895 (KZH79_25895)	5620517..5621005	-	489	WP_023104904.1	single-stranded DNA-binding protein	-
KZH79_RS25900 (KZH79_25900)	5621019..5621549	-	531	WP_121372660.1	DUF3158 family protein	-
KZH79_RS25905 (KZH79_25905)	5621555..5622283	-	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
KZH79_RS31730	5622440..5622952	-	513	WP_223697281.1	hypothetical protein	-
KZH79_RS25920 (KZH79_25920)	5622949..5624274	-	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	5510926..5637773	126847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (11-37)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T210556 WP_003134109.1 NZ_CP080011:5618900-5619310 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T210556 NZ_CP104539:c4295527-4295177 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8909.94 Da
• Isoelectric Point: 4.4230

>AT210556 WP_170869361.1 NZ_CP080011:5618658-5618900 [Pseudomonas aeruginosa]
MRCVVEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 243 bp

>AT210556 NZ_CP104539:c4295772-4295521 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA