Detailed information of TA system TA210555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4762036..4762622
• Replicon: chromosome
• Accession: NZ_CP080011
• Organism: Pseudomonas aeruginosa strain TL3773

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: KZH79_RS21875
• Protein ID: WP_003120987.1
• Coordinates: 4762036..4762335 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KZH79_RS21880
• Protein ID: WP_003448662.1
• Coordinates: 4762332..4762622 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZH79_RS21855 (KZH79_21855)	4757149..4757682	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
KZH79_RS21860 (KZH79_21860)	4757672..4758997	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
KZH79_RS21865 (KZH79_21865)	4759001..4760710	-	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
KZH79_RS21870 (KZH79_21870)	4760710..4761834	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
KZH79_RS21875 (KZH79_21875)	4762036..4762335	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZH79_RS21880 (KZH79_21880)	4762332..4762622	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
KZH79_RS21885 (KZH79_21885)	4762693..4763037	-	345	WP_016851612.1	hypothetical protein	-
KZH79_RS21890 (KZH79_21890)	4763178..4765154	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
KZH79_RS21895 (KZH79_21895)	4765151..4767040	-	1890	WP_196994395.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4703440..4790651	87211

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T210555 WP_003120987.1 NZ_CP080011:4762036-4762335 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T210555 NZ_CP104539:c4231220-4230879 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGAATCACTCTGGTCGATACCATCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT210555 WP_003448662.1 NZ_CP080011:4762332-4762622 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT210555 NZ_CP104539:c4231586-4231251 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCCGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCAGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: G8CP73