Detailed information of TA system TA210548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3516196..3517417
• Replicon: chromosome
• Accession: NZ_CP080007
• Organism: Pseudomonas aeruginosa strain S-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KXE03_RS16010
• Protein ID: WP_003160088.1
• Coordinates: 3516839..3517417 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KXE03_RS16005
• Protein ID: WP_023085114.1
• Coordinates: 3516196..3516750 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXE03_RS15980 (KXE03_15975)	3511764..3512315	+	552	WP_003450159.1	XRE family transcriptional regulator	-
KXE03_RS15985 (KXE03_15980)	3512312..3512710	-	399	WP_003117664.1	NADH-quinone oxidoreductase subunit A	-
KXE03_RS15990 (KXE03_15985)	3512723..3513046	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
KXE03_RS15995 (KXE03_15990)	3513377..3513838	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
KXE03_RS16000 (KXE03_15995)	3513843..3516038	+	2196	WP_124167498.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
KXE03_RS16005 (KXE03_16000)	3516196..3516750	+	555	WP_023085114.1	helix-turn-helix transcriptional regulator	Antitoxin
KXE03_RS16010 (KXE03_16005)	3516839..3517417	+	579	WP_003160088.1	HD domain-containing protein	Toxin
KXE03_RS16015 (KXE03_16010)	3517526..3518713	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KXE03_RS16020 (KXE03_16015)	3518703..3520874	-	2172	WP_034011389.1	type I secretion system permease/ATPase	-
KXE03_RS16025 (KXE03_16020)	3520864..3522141	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21816.65 Da
• Isoelectric Point: 5.1625

>T210548 WP_003160088.1 NZ_CP080007:3516839-3517417 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQCLAARRDA

Nucleotide

• Length: 579 bp

>T210548 NZ_CP104539:2732969-2733076 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT210548 WP_023085114.1 NZ_CP080007:3516196-3516750 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQSLARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT210548 NZ_CP104539:c2732922-2732856 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT