Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4015520..4016321 Replicon chromosome
Accession NZ_CP079993
Organism Escherichia coli strain EV36

Toxin (Protein)


Gene name yeeV Uniprot ID D8EBK0
Locus tag KYK84_RS19465 Protein ID WP_001094436.1
Coordinates 4015944..4016321 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7MN76
Locus tag KYK84_RS19460 Protein ID WP_015953067.1
Coordinates 4015520..4015897 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK84_RS19425 (4011432) 4011432..4012112 + 681 WP_001282927.1 WYL domain-containing protein -
KYK84_RS19430 (4012260) 4012260..4012937 + 678 WP_001097312.1 hypothetical protein -
KYK84_RS19435 (4012943) 4012943..4013176 + 234 WP_001278287.1 DUF905 family protein -
KYK84_RS19440 (4013266) 4013266..4014084 + 819 WP_001175142.1 DUF932 domain-containing protein -
KYK84_RS19445 (4014175) 4014175..4014660 + 486 WP_000860054.1 antirestriction protein -
KYK84_RS19450 (4014675) 4014675..4015151 + 477 WP_001186756.1 RadC family protein -
KYK84_RS19455 (4015220) 4015220..4015441 + 222 WP_000692312.1 DUF987 domain-containing protein -
KYK84_RS19460 (4015520) 4015520..4015897 + 378 WP_015953067.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KYK84_RS19465 (4015944) 4015944..4016321 + 378 WP_001094436.1 TA system toxin CbtA family protein Toxin
KYK84_RS19470 (4016318) 4016318..4016806 + 489 WP_000761714.1 DUF5983 family protein -
KYK84_RS19475 (4016818) 4016818..4017015 + 198 WP_000839254.1 DUF957 domain-containing protein -
KYK84_RS19480 (4017100) 4017100..4017945 + 846 WP_001280513.1 DUF4942 domain-containing protein -
KYK84_RS19485 (4018016) 4018016..4019551 + 1536 WP_000492897.1 EAL domain-containing protein -
KYK84_RS19490 (4019948) 4019948..4020118 + 171 Protein_3826 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papG / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE 3964796..4157120 192324
- flank IS/Tn - - 4020014..4020118 104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13958.84 Da        Isoelectric Point: 6.8603

>T210542 WP_001094436.1 NZ_CP079993:4015944-4016321 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Download         Length: 378 bp

>T210542 NZ_CP104539:1085546-1085881 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13766.60 Da        Isoelectric Point: 6.2021

>AT210542 WP_015953067.1 NZ_CP079993:4015520-4015897 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Download         Length: 378 bp

>AT210542 NZ_CP104539:1085298-1085546 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2L1N0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z3CIS0

References