Detailed information of TA system TA021054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3222663..3223291
• Replicon: chromosome
• Accession: NC_005823
• Organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: LIC_RS13620
• Protein ID: WP_000281042.1
• Coordinates: 3222893..3223291 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q72P17
• Locus tag: LIC_RS13615
• Protein ID: WP_001192484.1
• Coordinates: 3222663..3222893 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIC_RS13585	3218264..3218473	-	210	WP_000041838.1	hypothetical protein	-
LIC_RS13590	3218605..3219234	+	630	WP_001081629.1	helix-turn-helix domain-containing protein	-
LIC_RS13595	3219441..3220364	+	924	WP_000680714.1	M28 family peptidase	-
LIC_RS13600	3220623..3221312	+	690	WP_000590460.1	SH3 domain-containing protein	-
LIC_RS13605	3221515..3221844	+	330	WP_000611884.1	hypothetical protein	-
LIC_RS13610	3221841..3222371	+	531	WP_000129981.1	hypothetical protein	-
LIC_RS13615	3222663..3222893	+	231	WP_001192484.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LIC_RS13620	3222893..3223291	+	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
LIC_RS13625	3223389..3223691	+	303	WP_000479559.1	hypothetical protein	-
LIC_RS13635	3223746..3224893	+	1148	WP_100225665.1	IS3-like element IS1500A family transposase	-
LIC_RS13640	3224948..3225175	+	228	WP_000130601.1	hypothetical protein	-
LIC_RS13645	3225318..3226394	+	1077	WP_001170562.1	hypothetical protein	-
LIC_RS13650	3226395..3226853	+	459	WP_001239008.1	GNAT family N-acetyltransferase	-
LIC_RS13655	3227070..3227930	-	861	WP_000682236.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T21054 WP_000281042.1 NC_005823:3222893-3223291 [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T21054 NC_005823:3222893-3223291 [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130]
ATGTATCTTTTGGATACCAATATTTGTATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGCGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8698.99 Da
• Isoelectric Point: 9.8940

>AT21054 WP_001192484.1 NC_005823:3222663-3222893 [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT21054 NC_005823:3222663-3222893 [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTTATCCAAAAAGTTGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGGGAGAGAACAACCGAAATCTGATAAAAGAGAAAAACTTTAA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6

Antitoxin

• AlphaFold DB: A0A0E2D7U1