Detailed information of TA system TA210538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3594622..3596059
• Replicon: chromosome
• Accession: NZ_CP079993
• Organism: Escherichia coli strain EV36

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: KYK84_RS17455
• Protein ID: WP_000155570.1
• Coordinates: 3594622..3595695 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: KYK84_RS17460
• Protein ID: WP_000461704.1
• Coordinates: 3595688..3596059 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYK84_RS17440 (3590055)	3590055..3592244	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
KYK84_RS17445 (3592241)	3592241..3593857	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
KYK84_RS17450 (3594217)	3594217..3594480	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
KYK84_RS17455 (3594622)	3594622..3595695	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
KYK84_RS17460 (3595688)	3595688..3596059	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
KYK84_RS17465 (3596414)	3596414..3597277	+	864	WP_000787597.1	GTPase family protein	-
KYK84_RS17470 (3597369)	3597369..3598190	+	822	WP_000197391.1	DUF932 domain-containing protein	-
KYK84_RS17475 (3598407)	3598407..3599108	+	702	WP_000189409.1	WYL domain-containing protein	-
KYK84_RS17480 (3599149)	3599149..3599385	+	237	WP_001144029.1	protein YpjK	-
KYK84_RS17485 (3599385)	3599385..3599828	+	444	WP_000824222.1	lipoprotein YfjS	-
KYK84_RS17490 (3599852)	3599852..3600319	+	468	WP_000702495.1	protein YfjT	-
KYK84_RS17495 (3600706)	3600706..3600858	-	153	Protein_3436	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T210538 WP_000155570.1 NZ_CP079993:3594622-3595695 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T210538 NZ_CP104539:280312-280542 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT210538 WP_000461704.1 NZ_CP079993:3595688-3596059 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT210538 NZ_CP104539:280539-280898 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• AlphaFold DB: A0A373F4G4