Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2931411..2932242 Replicon chromosome
Accession NZ_CP079993
Organism Escherichia coli strain EV36

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag KYK84_RS14350 Protein ID WP_000854814.1
Coordinates 2931868..2932242 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag KYK84_RS14345 Protein ID WP_001285584.1
Coordinates 2931411..2931779 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK84_RS14330 (2929078) 2929078..2930610 + 1533 WP_001350525.1 protein YeeR -
KYK84_RS14335 (2930607) 2930607..2931053 + 447 WP_000187523.1 RadC family protein -
KYK84_RS14340 (2931116) 2931116..2931337 + 222 WP_000692323.1 DUF987 domain-containing protein -
KYK84_RS14345 (2931411) 2931411..2931779 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KYK84_RS14350 (2931868) 2931868..2932242 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KYK84_RS14355 (2932239) 2932239..2932433 + 195 WP_000988600.1 DUF5983 family protein -
KYK84_RS14360 (2932479) 2932479..2932559 + 81 Protein_2819 hypothetical protein -
KYK84_RS14365 (2932848) 2932848..2932976 - 129 Protein_2820 transposase domain-containing protein -
KYK84_RS14370 (2933096) 2933096..2933230 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
KYK84_RS14375 (2933331) 2933331..2933660 - 330 WP_000450409.1 DUF496 family protein -
KYK84_RS14380 (2933832) 2933832..2934890 - 1059 WP_001200891.1 FUSC family protein -
KYK84_RS14385 (2935088) 2935088..2935561 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
KYK84_RS14390 (2935680) 2935680..2936846 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T210536 WP_000854814.1 NZ_CP079993:2931868-2932242 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T210536 NZ_CP104536:c4084050-4083700 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT210536 WP_001285584.1 NZ_CP079993:2931411-2931779 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT210536 NZ_CP104536:c4084295-4084044 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References