Detailed information of TA system TA210525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2363914..2364552
• Replicon: chromosome
• Accession: NZ_CP079993
• Organism: Escherichia coli strain EV36

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KYK84_RS11460
• Protein ID: WP_000813794.1
• Coordinates: 2363914..2364090 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KYK84_RS11465
• Protein ID: WP_001270286.1
• Coordinates: 2364136..2364552 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYK84_RS11440 (2359533)	2359533..2360708	-	1176	WP_001236265.1	BenE family transporter YdcO	-
KYK84_RS11445 (2360800)	2360800..2361336	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KYK84_RS11450 (2361409)	2361409..2363370	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KYK84_RS11455 (2363462)	2363462..2363692	-	231	WP_000494244.1	YncJ family protein	-
KYK84_RS11460 (2363914)	2363914..2364090	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KYK84_RS11465 (2364136)	2364136..2364552	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KYK84_RS11470 (2364631)	2364631..2366037	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KYK84_RS11475 (2366282)	2366282..2367427	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KYK84_RS11480 (2367445)	2367445..2368458	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KYK84_RS11485 (2368459)	2368459..2369400	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T210525 WP_000813794.1 NZ_CP079993:2363914-2364090 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T210525 NZ_CP104536:870991-871398 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT210525 WP_001270286.1 NZ_CP079993:2364136-2364552 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT210525 NZ_CP104536:870744-871010 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6HPB