Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1371327..1371945 Replicon chromosome
Accession NZ_CP079993
Organism Escherichia coli strain EV36

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KYK84_RS06650 Protein ID WP_001291435.1
Coordinates 1371327..1371545 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KYK84_RS06655 Protein ID WP_000344800.1
Coordinates 1371571..1371945 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK84_RS06615 (1366616) 1366616..1367188 + 573 WP_000779842.1 YbaY family lipoprotein -
KYK84_RS06620 (1367219) 1367219..1367530 - 312 WP_000409911.1 MGMT family protein -
KYK84_RS06630 (1367909) 1367909..1368262 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KYK84_RS06635 (1368304) 1368304..1369854 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KYK84_RS06640 (1370018) 1370018..1370488 - 471 WP_000136192.1 YlaC family protein -
KYK84_RS06645 (1370604) 1370604..1371155 - 552 WP_000102564.1 maltose O-acetyltransferase -
KYK84_RS06650 (1371327) 1371327..1371545 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KYK84_RS06655 (1371571) 1371571..1371945 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KYK84_RS06660 (1372491) 1372491..1375640 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KYK84_RS06665 (1375663) 1375663..1376856 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210524 WP_001291435.1 NZ_CP079993:c1371545-1371327 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210524 NZ_CP104536:733710-734006 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210524 WP_000344800.1 NZ_CP079993:c1371945-1371571 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210524 NZ_CP104536:734008-734403 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References